Nezhad Sistani Maryam, Maleki Anahid, Salimi Maryam, Ghaffari Novin Marefat, Nazarian Hamid
Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Anesthesiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Int J Reprod Biomed. 2017 Aug;15(8):471-478.
common use of sevoflurane in congenital defects during repeated surgeries may have detrimental effects on spermatogenesis after puberty.
This study investigated sevoflurane effects on spermatogenesis process in male mature mice after exposure in prepubertal time.
24 neonatal NMRI male mice were randomly classified in three groups. Experimental 1 and 2 groups (exposure to 1 minimum alveolar concentration (MAC) and 2 MAC sevoflurane, respectively in 2 lit/min oxygen (O) for 7 days (30 min, daily) and control. All groups were sacrificed after 2 months. Histological assessment, immunohistochemistry and apoptosis process was done. Bax and Bcl expression was evaluated in the testicular tissue by real time Poly Chain Reaction
Our results showed that the integrity of testicular tissue was preserved in both experimental groups. Count of spermatogonial cells had significant decrease in group 2 compared to others. The rate of apoptosis in spermatogonial cells was 15±3% and 9±2% in the group 2 and 1, respectively. Also, Bax/Bcl ratio was 0.2615, 1.0070 and 9.3657 in control, experimental group 1 and 2, respectively. This result was significant (p≤0.002) between groups 2 with other groups.
Continuous exposure of 2 MAC sevoflurane in 2 lit/min O simultaneous during prepubertal may create more testicular tissue damage in terms of cellular and molecular function compared to continuous exposure to lower level of sevoflurane by increase in ratio of Bax/Bcl and apoptosis in germ cells after puberty.
在先天性缺陷患儿多次手术中常用的七氟醚可能对青春期后的精子发生产生有害影响。
本研究调查了青春期前暴露于七氟醚对雄性成年小鼠精子发生过程的影响。
将24只新生NMRI雄性小鼠随机分为三组。实验1组和2组（分别在2升/分钟氧气（O）中暴露于1最低肺泡浓度（MAC）和2 MAC七氟醚，每天30分钟，持续7天）和对照组。2个月后处死所有组。进行组织学评估、免疫组织化学和凋亡过程检测。通过实时聚合酶链反应评估睾丸组织中Bax和Bcl的表达。
我们的结果表明，两个实验组的睾丸组织完整性均得以保留。与其他组相比，第2组精原细胞数量显著减少。第2组和第1组精原细胞的凋亡率分别为15±3%和9±2%。此外，对照组、实验组1和2的Bax/Bcl比值分别为0.2615、1.0070和9.3657。第2组与其他组之间的这一结果具有显著性（p≤0.002）。
与青春期前持续暴露于较低水平的七氟醚相比，青春期前在2升/分钟氧气中持续暴露于2 MAC七氟醚可能通过增加Bax/Bcl比值和青春期后生殖细胞凋亡，在细胞和分子功能方面造成更多睾丸组织损伤。
