Department of Anesthesiology and Reanimation, Gaziosmanpasa University School of Medicine, Tokat, Turkey.
Inhal Toxicol. 2013 Mar;25(4):192-8. doi: 10.3109/08958378.2013.773109.
Evaluation of inhalation anesthetics on sperm and reproductive hormones are extremely important.
Investigation of the effects of sevoflurane used as an inhalation anesthetic on sperm morphology and reproductive hormones in rat testes.
Forty Wistar-Albino male rats were divided into five groups of eight rats each. The control group received 2 L/min oxygen for seven days, 2 h/day while sevoflurane treatment S1 received 1 minimal alveolar concentration (MAC) sevoflurane + 2 L/min oxygen for seven days, 2 h/day, and sevoflurane S2 received 1 MAC sevoflurane + 2 L/min oxygen for seven days, 2 h/day followed by seven days of no treatment. Sevoflurane treatment S3 received 1 MAC sevoflurane + 2 L/min oxygen for 14 days, 2 h/day and sevoflurane treatment S4 received 1 MAC sevoflurane + 2 L/min oxygen for 14 days, 2 h/day, with no treatment for the following seven days. All rats were examined histologically after experimental procedures. Rat luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone (T), and inhibin levels were measured.
Histological injury scores were significantly higher in S2, S3, and S4 receiving sevoflurane in comparison to the control group (p = 0.001, <0.001, and 0.001, respectively). Sperm motility and concentration decreased in S3 and S4 compared to the control group (p = 0.03 and 0.02, respectively). Significant differences were detected among all groups for serum LH, FSH, T, and inhibin serum concentrations (p < 0.05).
Testicular and sperm morphology, and reproductive hormones were affected by chronic exposure to sevoflurane. However, more randomized, controlled, and well-designed clinical studies with larger population are needed to confirm of these results.
评估吸入麻醉剂对精子和生殖激素的影响非常重要。
研究七氟醚作为吸入麻醉剂对大鼠睾丸精子形态和生殖激素的影响。
将 40 只 Wistar-Albino 雄性大鼠分为五组，每组 8 只。对照组每天接受 2 L/min 氧气，共 7 天，每天 2 小时；七氟醚处理 S1 组接受 1 最小肺泡浓度（MAC）七氟醚+2 L/min 氧气，共 7 天，每天 2 小时；七氟醚处理 S2 组接受 1 MAC 七氟醚+2 L/min 氧气，共 7 天，每天 2 小时，然后不治疗 7 天；七氟醚处理 S3 组接受 1 MAC 七氟醚+2 L/min 氧气，共 14 天，每天 2 小时；七氟醚处理 S4 组接受 1 MAC 七氟醚+2 L/min 氧气，共 14 天，每天 2 小时，然后不治疗 7 天。所有大鼠在实验程序结束后进行组织学检查。测量大鼠黄体生成素（LH）、卵泡刺激素（FSH）、睾酮（T）和抑制素水平。
与对照组相比，接受七氟醚处理的 S2、S3 和 S4 组的组织学损伤评分显著升高（p=0.001、<0.001 和 0.001）。与对照组相比，S3 和 S4 组的精子活力和浓度降低（p=0.03 和 0.02）。所有组之间血清 LH、FSH、T 和抑制素血清浓度均存在显著差异（p<0.05）。
慢性暴露于七氟醚会影响睾丸和精子形态以及生殖激素。然而，需要更多的随机、对照和精心设计的临床研究，以确认这些结果。
