Dolman Nick J, Samson Brent A, Chambers Kevin M, Janes Michael S, Mandavilli Bhaskar S
Thermo Fisher Scientific, 100 Technology Drive, Pittsburgh, PA, 15219, USA.
Thermo Fisher Scientific, 29851 Willow Creek Road, Eugene, OR, 97402, USA.
Methods Mol Biol. 2018;1683:59-71. doi: 10.1007/978-1-4939-7357-6_5.
Macroautophagy, hereafter referred to as autophagy, is a predominately pro-survival catabolic process responsible for the degradation of long-lived or aggregated proteins, invading microorganisms and damaged or redundant intracellular organelles. Removal of these entities is achieved through encompassment of the target by the autophagosome and subsequent delivery to the lysosome. The use of fluorescence microscopy is a common method to investigate autophagy through monitoring the spatial and temporal recruitment both of autophagosomal markers and cargo to the autophagosome. In this section, we will discuss the use of high content imaging (HCI) and analysis in the study of autophagy with reference to commonly used markers of autophagosomal formation.
