Muhammad Sohail, Xu Guanhong, Wei Fangdi, Ma Yujie, Ma Yunsu, Song Yueyue, Shi Menglan, Xu Xiaoman, Cen Yao, Hu Qin
School of Pharmacy, Nanjing Medical University, Nanjing 211166, Jiangsu, China.
Nanomaterials (Basel). 2017 Oct 30;7(11):358. doi: 10.3390/nano7110358.
An analytical technique based on fluorescence quenching of CdTe/CdS/ZnS quantum dots (QDs) was developed to quantify verapamil in commercially available preparations. Various reaction parameters were optimized and the method developed was validated. One way analysis of variance (ANOVA) and post hoc tests at a 5% significance level were performed to justify the significance of the variation in observations. The linear range of the verapamil concentration was 0.25-5 µg/mL while the limit of detection was 20 µg/mL. Recovery and relative standard deviations were not more than ±10% of the actual amount and <5.9%, respectively. Foreign materials, common metal ions and pharmaceutical excipients of dosage forms caused little interference. To verify the application of the analytical method, the quantity of verapamil in commercially available dosage forms was measured. Verapamil content in the tablets and injections was not more than ±10% of the stated amount and it conformed to the specifications of both the British and the United States pharmacopoeias. In the case of statistical analysis, -value was <0.05 in almost all levels of all parameters except for the optimized level of system. It can be concluded from the results that the designed method is simple, reliable, cost effective, selective, rapid and sensitive enough to be used for quantitative measurement of the verapamil HCl in dosage forms for quality control purposes.
开发了一种基于CdTe/CdS/ZnS量子点（QDs）荧光猝灭的分析技术，用于定量市售制剂中的维拉帕米。优化了各种反应参数，并对所开发的方法进行了验证。进行了单因素方差分析（ANOVA）和5%显著性水平的事后检验，以证明观测值变化的显著性。维拉帕米浓度的线性范围为0.25 - 5 µg/mL，而检测限为20 µg/mL。回收率和相对标准偏差分别不超过实际量的±10%和<5.9%。剂型中的杂质、常见金属离子和药用辅料几乎不产生干扰。为验证该分析方法的应用，测定了市售剂型中维拉帕米的含量。片剂和注射剂中维拉帕米的含量不超过规定量的±10%，符合英国和美国药典的规格。在统计分析中，除系统优化水平外，几乎所有参数的所有水平的P值均<0.05。从结果可以得出结论，所设计的方法简单、可靠、经济高效、选择性好、快速且灵敏，足以用于剂型中盐酸维拉帕米的定量测定以进行质量控制。
