Neve R L, Finch E A, Dawes L R
Division of Genetics, Children's Hospital, Boston, Massachusetts 02115.
Neuron. 1988 Oct;1(8):669-77. doi: 10.1016/0896-6273(88)90166-3.
An alternate form of the Alzheimer amyloid protein precursor mRNA that encodes a protease inhibitor domain has recently been reported. Oligonucleotide probes that differentiate between the two mRNAs are used to describe the expression of each amyloid precursor transcript in the human brain. RNA blot analyses show that one of the mRNAs is expressed selectively in the nervous system, that the two messages display different regional distributions in the adult human brain, and that the expression of the two mRNAs is differentially affected in Down's syndrome brain and in Alzheimer's disease frontal cortex. In situ hybridization shows that the two transcripts display the same laminar distribution in the adult cortex but that the transcripts differ significantly in their levels of expression in pyramidal cells of the hippocampus.
最近报道了一种阿尔茨海默病淀粉样蛋白前体mRNA的替代形式，它编码一个蛋白酶抑制剂结构域。用于区分这两种mRNA的寡核苷酸探针被用来描述每种淀粉样前体转录本在人脑中的表达情况。RNA印迹分析表明，其中一种mRNA在神经系统中选择性表达，这两种信息在成人大脑中显示出不同的区域分布，并且这两种mRNA的表达在唐氏综合征大脑和阿尔茨海默病额叶皮质中受到不同的影响。原位杂交表明，这两种转录本在成人皮质中显示出相同的层状分布，但在海马体锥体细胞中的表达水平有显著差异。
