Naz Shazia, Shoaib Muhammad Harris, Bashir Lubna, Yousuf Rabia Ismail, Anjum Fakhsheena, Siddiqui Fahad, Yaseen Saima
Department of Pharmaceutics, Faculty of Pharmacy, Federal Urdu University of Arts, Science and Technology, Karachi, Pakistan.
Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan.
Pak J Pharm Sci. 2017 Sep;30(5):1645-1649.
Cefaclor was analyzed in the human plasma by developing a simple, precise and accurate assay method which was then validated for its accuracy, specificity and precision. The mobile phase comprised of a mixture of sodium 1-pentanesulfonate, water, triethylamine and methanol. Phosphoric acid was used to adjust the pH to 2.5±0.1. The flow rate was maintained at 1.5ml/min and the wavelength was set at 265 nm. A C-18 HPLC, column 5um particle size, L x 1.D. 25cm x 4.6mm (Supelcosil) was utilized for chromatographic separation. The retention time of Cefaclor was found to be 17min. This method was validated for selectivity, accuracy, precision, repeatability, reproducibility, recovery, linearity, and stability. Calibration curves were found linear were in the range of 0.39µg/ml to50µg/mland the coefficient of correlation (R) was found to be 0.999. Hence, this method has been found useful for the determination of Cefaclor in plasma.
通过开发一种简单、精确且准确的测定方法，对人血浆中的头孢克洛进行分析，并对该方法的准确性、特异性和精密度进行验证。流动相由1-戊烷磺酸钠、水、三乙胺和甲醇的混合物组成。用磷酸将pH值调至2.5±0.1。流速保持在1.5ml/min，波长设定为265nm。使用粒径为5μm、长×内径为25cm×4.6mm的C-18高效液相色谱柱（Supelcosil）进行色谱分离。发现头孢克洛的保留时间为17分钟。该方法在选择性、准确性、精密度、重复性、再现性、回收率、线性和稳定性方面得到验证。校准曲线在0.39μg/ml至50μg/ml范围内呈线性，相关系数（R）为0.999。因此，已发现该方法可用于测定血浆中的头孢克洛。
