Zhou Sitong, Huang Yu-Shan, Kingsley Paul D, Cyr Kathryn H, Palis James, Wan Jiandi
Microsystems Engineering, Rochester Institute of Technology, Rochester, New York 14623, USA.
Department of Biomedical Genetics, University of Rochester, Rochester, New York 14642, USA.
Biomicrofluidics. 2017 Oct 23;11(5):054112. doi: 10.1063/1.4999949. eCollection 2017 Sep.
Primitive erythroblasts (precursors of red blood cells) enter vascular circulation during the embryonic period and mature while circulating. As a result, primitive erythroblasts constantly experience significant hemodynamic shear stress. Shear-induced deformation of primitive erythroblasts however, is poorly studied. In this work, we examined the deformability of primitive erythroblasts at physiologically relevant flow conditions in microfluidic channels and identified the regulatory roles of the maturation stage of primitive erythroblasts and cytoskeletal protein 4.1 R in shear-induced cell deformation. The results showed that the maturation stage affected the deformability of primitive erythroblasts significantly and that primitive erythroblasts at later maturational stages exhibited a better deformability due to a matured cytoskeletal structure in the cell membrane.
原始红细胞（红细胞的前体）在胚胎期进入血管循环并在循环过程中成熟。因此，原始红细胞不断经历显著的血液动力学剪切应力。然而，剪切诱导的原始红细胞变形研究较少。在这项工作中，我们在微流控通道中生理相关的流动条件下研究了原始红细胞的变形能力，并确定了原始红细胞成熟阶段和细胞骨架蛋白4.1R在剪切诱导的细胞变形中的调节作用。结果表明，成熟阶段显著影响原始红细胞的变形能力，并且后期成熟阶段的原始红细胞由于细胞膜中成熟的细胞骨架结构而表现出更好的变形能力。
