Ghaffar Abdul, Yameen Muhammad, Aslam Nosheen, Jalal Fatima, Noreen Razia, Munir Bushra, Mahmood Zahed, Saleem Sadaf, Rafiq Naila, Falak Sadia, Tahir Imtiaz Mahmood, Noman Muhammad, Farooq Muhammad Umar, Qasim Samina, Latif Farooq
Department of Biochemistry, Government College University, Faisalabad, 38000, Pakistan.
Department of Zoology and Fisheries, Government College University, Faisalabad, 38000, Pakistan.
Chem Cent J. 2017 Oct 2;11(1):97. doi: 10.1186/s13065-017-0331-z.
The plant biomass and agro-industrial wastes show great potential for their use as attractive low cost substrates in biotechnological processes. Wheat straw and corn cob as hemicellulosic substrates were acid hydrolyzed and enzymatically saccharified for high xylose production. The hydrolysate was concentrated and fermented by using Saccharomyces cerevisiae and Kluyveromyces for production of xylitol.
Acid hydrolysis of wheat straw and corn cob in combination with enzymatic hydrolysis showed great potential for production of free sugars from these substrates. Kluyveromyces produced maximum xylitol from acid treated wheat straw residues with enzymatic saccharification. The percentage xylitol yield was 89.807 g/L and volumetric productivity of 0.019 g/L/h. Kluyveromyces also produced maximum xylitol from corn cob acid hydrolyzed liquor with xylitol yield 87.716 g/L and volumetric productivity 0.018 g/L/h.
Plant and agro-industrial biomass can be used as a carbohydrate source for the production of xylitol and ethanol after microbial fermentation. This study revealed that wheat straw acid and enzyme hydrolyzed residue proved to be best raw material for production of xylitol with S. cerevisiae. The xylitol produced can be utilized in pharmaceuticals after purification on industrial scale as pharmaceutical purposes.
植物生物质和农业工业废弃物在生物技术过程中作为有吸引力的低成本底物具有巨大潜力。麦秸和玉米芯作为半纤维素底物经过酸水解和酶糖化以高产木糖。水解产物经浓缩后，使用酿酒酵母和克鲁维酵母进行发酵以生产木糖醇。
麦秸和玉米芯的酸水解与酶水解相结合显示出从这些底物生产游离糖的巨大潜力。克鲁维酵母从经酸处理的麦秸残渣酶糖化产物中产生的木糖醇最多。木糖醇产量百分比为89.807 g/L，体积生产率为0.019 g/L/h。克鲁维酵母从玉米芯酸水解液中也产生了最多的木糖醇，木糖醇产量为87.716 g/L，体积生产率为0.018 g/L/h。
植物和农业工业生物质可作为碳水化合物来源，经微生物发酵后用于生产木糖醇和乙醇。本研究表明，麦秸酸酶水解残渣被证明是用酿酒酵母生产木糖醇的最佳原料。所生产的木糖醇经工业规模纯化后可用于制药目的。
