嘌呤能P2X7受体功能基因多态性与中国绝经后女性的肥胖易感性相关。
Purinergic P2X7 receptor functional genetic polymorphisms are associated with the susceptibility to obesity in Chinese postmenopausal women.
作者信息
Liu Xingzi, Rao Shenqiang, Gong Chengxin, Li Tao, Ding Lu, Wang Shuo, Zhong Peipei, Wang Jihong, Zhao Jiani, Zhou Yuru, Xiong Chaopeng, Tu Yunming, Liang Shangdong, Xu Hong
机构信息
Undergraduate Student of Clinical Medical College, JiangXi Medical College of Nanchang University, Nanchang, Jiangxi, P.R. China.
Department of Physiology, JiangXi Medical College of Nanchang University, Nanchang, Jiangxi, P.R. China.
出版信息
Menopause. 2018 Mar;25(3):329-335. doi: 10.1097/GME.0000000000000991.
OBJECTIVE
We conducted a case-control study to investigate the associations of functional single-nucleotide polymorphisms in the purinergic P2X7 receptor (P2X7R) gene (rs2393799, rs7958311, rs1718119, rs2230911, and rs3751143) with obesity and overweight in a population of Chinese postmenopausal women.
METHODS
Our study included 180 obese women, 179 overweight women, and 204 controls. All participants were genotyped at the P2X7R rs2393799, rs7958311, rs1718119, rs2230911, and rs3751143 loci via allele-specific polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism procedures. The relationships between P2X7R genetic polymorphisms and their associated haplotypes with obesity (body mass index [BMI] ≥30 kg/m] and overweight (25 kg/m ≤ BMI < 30 kg/m) were evaluated.
RESULTS
Our results showed that P2X7R rs2230911G and rs1718119A were associated with an increased risk of obesity; in particular, both carriers of the rs2230911G allele and GG/(CG + GG) genotypes (G vs C, P < 0.001, odds ratio [OR] 2.87, 95% confidence interval [CI] 1.98-4.16; GG vs CC, P < 0.001, OR 8.76, 95% CI 3.29-23.35; CG + GG vs CC, P < 0.001, OR 2.54, 95% CI 1.63-3.95) and carriers of the rs17181191A allele and GA/(GA + AA) genotypes (A vs G, P < 0.001, OR 2.97, 95% CI 1.86-4.74; GA vs GG, P = 0.001, OR 2.72, 95% CI 1.55-4.79; GA + AA vs GG, P < 0.001, OR 3.05, 95% CI 1.79-5.19) were at a higher risk of obesity. No association with obesity or overweight was observed for the other three P2X7R polymorphisms (rs2393799, rs7958311, and rs3751143). Haplotype analysis indicated that P2X7R rs1718119A-rs2230911G-rs3751143C appeared to be a significant risk haplotype with obesity (P = 0.0005, OR 2.37, 95% CI 1.45-3.90).
CONCLUSIONS
P2X7R functional genetic polymorphisms and their estimated haplotypes are associated with obesity in Chinese postmenopausal women.
目的
我们开展了一项病例对照研究，以调查嘌呤能P2X7受体（P2X7R）基因（rs2393799、rs7958311、rs1718119、rs2230911和rs3751143）中的功能性单核苷酸多态性与中国绝经后女性人群肥胖及超重之间的关联。
方法
我们的研究纳入了180名肥胖女性、179名超重女性和204名对照。所有参与者均通过等位基因特异性聚合酶链反应（PCR）和PCR-限制性片段长度多态性方法，对P2X7R基因的rs2393799、rs7958311、rs1718119、rs2230911和rs3751143位点进行基因分型。评估了P2X7R基因多态性及其相关单倍型与肥胖（体重指数[BMI]≥30kg/m²）和超重（25kg/m²≤BMI＜30kg/m²）之间的关系。
结果
我们的结果显示，P2X7R基因的rs2230911G和rs1718119A与肥胖风险增加相关；具体而言，rs2230911G等位基因携带者以及GG/（CG + GG）基因型（G与C相比，P＜0.001，比值比[OR]2.87，95%置信区间[CI]1.98 - 4.16；GG与CC相比，P＜0.001，OR 8.76，95% CI 3.29 - 23.35；CG + GG与CC相比，P＜0.001，OR 2.54，95% CI 1.63 - 3.95），以及rs1718119A等位基因携带者和GA/（GA + AA）基因型（A与G相比，P＜0.001，OR 2.97，95% CI 1.86 - 4.74；GA与GG相比，P = 0.001，OR 2.72，95% CI 1.55 - 4.79；GA + AA与GG相比，P＜0.001，OR 3.05，95% CI 1.79 - 5.19）肥胖风险更高。未观察到其他三个P2X7R多态性（rs2393799、rs7958311和rs3751143）与肥胖或超重有关。单倍型分析表明，P2X7R基因的rs1718119A-rs2230911G-rs3751143C似乎是与肥胖相关的显著风险单倍型（P = 0.0005，OR 2.37，95% CI 1.45 - 3.90）。
结论
P2X7R功能性基因多态性及其估计的单倍型与中国绝经后女性肥胖相关。
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