NTT Basic Research Laboratories, NTT Corporation , 3-1 Morinosato Wakamiya, Atsugi, Kanagawa 243-0198, Japan.
Langmuir. 2017 Nov 21;33(46):13277-13283. doi: 10.1021/acs.langmuir.7b02156. Epub 2017 Nov 9.
The localization of liquid-ordered (L) and liquid-crystalline (L) phase domains on a silicon substrate with a microwell array is investigated. Although the phase separation of the L and L phases on both a giant unilamellar vesicle (GUV) and a supported membrane remains stable for a long time, the lateral diffusion of lipids across each domain boundary occurs quickly. Since the phase separation and domain arrangement are governed by the stiffness and lateral tension of the lipid membrane, the phase separation is rearranged on a micropatterned substrate. Similar phase separation of the L and L phases is observed at a lipid membrane suspended over a microwell. However, the L phase is preferred at a suspended membrane, and saturated lipids and cholesterol are excluded toward the supporting membrane on the periphery. Since the L domain area is reduced by anisotropic diffusion through the boundary between the suspended and supported membranes, a very slow reduction rate with a linear functional relation is observed. Finally, a localized L phase domain is observed at a membrane suspended over a microwell, which is surrounded by an L phase supported membrane.
在具有微井阵列的硅衬底上对液有序（L）相和液晶（L）相畴的定位进行了研究。尽管在巨大的单层囊泡（GUV）和支撑膜上，L 相和 L 相的相分离在很长一段时间内仍然稳定，但脂质在各畴边界的横向扩散很快发生。由于相分离和畴排列受脂质膜的刚度和横向张力的控制，因此相分离在微图案化衬底上重新排列。在悬于微井上方的脂质膜中观察到类似的 L 相和 L 相的相分离。然而，在悬浮膜中更喜欢 L 相，饱和脂质和胆固醇被排斥到外围的支撑膜上。由于 L 畴区域通过悬浮和支撑膜之间的边界进行各向异性扩散而减少，因此观察到非常缓慢的减少率与线性函数关系。最后，在悬于微井上方的膜中观察到局部 L 相畴，该畴被支撑的 L 相膜包围。
