Ohkawa H, Mikata A, Harigaya K, Ueda R
Department of Pathology, School of Medicine, Keio University, Tokyo, Japan.
Exp Hematol. 1989 Jan;17(1):30-3.
Lymphoid stromal (SG) cells have been isolated from the lymph node of a patient with malignant lymphoma, and characterized by positive reaction with a monoclonal antibody against the T-zone stromal cells in human lymph nodes. B-acute lymphoblastic leukemia (BALL) cells showed prominent emperipolesis toward SG cells when they were cocultured, whereas T-acute lymphoblastic leukemia (TALL) cells attached firmly to the surface of SG cells. Autologous peripheral B and T cells behaved, respectively, in the same way as BALL and TALL cells. Both BALL and TALL cells while directly interacting with the SG cells were completely inhibited from incorporating [3H]thymidine, although radioactive grains were observed in 16.4%-12.4% of supernatant BALL and 13.8%-13.0% of supernatant TALL cells in each coculture. Furthermore the media conditioned by SG cells significantly increased the incorporation of [3H]thymidine into the TALL cells as much as 190% of the control. These results indicate that SG cells undergo tissue-specific cellular interactions with B- and T-lymphoid cell lines but not with a myeloid cell line, and they can modify their growth by two distinct mechanisms. SG cells proved to be very useful in studying the effect of the lymphoid microenvironment on the proliferation of lymphocytes in vivo.
已从一名恶性淋巴瘤患者的淋巴结中分离出淋巴样基质（SG）细胞，并通过与抗人淋巴结T区基质细胞的单克隆抗体呈阳性反应进行表征。当B急性淋巴细胞白血病（BALL）细胞与SG细胞共培养时，对SG细胞表现出明显的血细胞吞噬作用，而T急性淋巴细胞白血病（TALL）细胞则牢固地附着在SG细胞表面。自体外周血B细胞和T细胞的行为分别与BALL细胞和TALL细胞相同。尽管在每种共培养物的上清液BALL细胞的16.4%-12.4%和上清液TALL细胞的13.8%-13.0%中观察到放射性颗粒，但BALL细胞和TALL细胞在与SG细胞直接相互作用时，掺入[3H]胸腺嘧啶核苷的过程均被完全抑制。此外，SG细胞条件培养基使TALL细胞中[3H]胸腺嘧啶核苷的掺入量显著增加，高达对照的190%。这些结果表明，SG细胞与B淋巴细胞系和T淋巴细胞系发生组织特异性细胞相互作用，但不与髓细胞系发生这种相互作用，并且它们可以通过两种不同机制调节自身生长。SG细胞被证明在研究淋巴微环境对体内淋巴细胞增殖的影响方面非常有用。
