Huang Minlu, Ma Xinjie, Shi Hongpeng, Hu Lei, Fan Zhiyuan, Pang Li, Zhu Fan, Yang Xiao, Xu Wei, Liu Binya, Zhu Zhenggang, Li Chen
Shanghai Key Laboratory of Gastric Neoplasms, Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People's Republic of China.
Oncotarget. 2017 Aug 10;8(43):74479-74493. doi: 10.18632/oncotarget.20157. eCollection 2017 Sep 26.
, a microtubule-associated protein (MAP), is overexpressed in diverse types of human cancer. The expression and critical role of in human gastric cancer (GC), however, remains largely unknown. Here, we conducted molecular, cellular and clinical analyses to evaluate the functional link of to GC. expression was elevated in gastric tumors, and its expression strongly correlated with lymph node metastasis and TNM stage. In addition, over-expression of in GC cell lines enhanced cell proliferation, cycle progression, migration, invasion, as well as tumor growth and metastatic dissemination . Furthermore, exhibited a strong cell cycle correlated expression. The knockdown of inhibited the regrowth of microtubules in GC cells. FAM83D was co-immunoprecipitated with HMMR, TPX2, and AURKA, a set of drivers of mitosis progression. Taken together, our results demonstrate as an important player in the development of human gastric cancer, and as a potential therapeutic target for the treatment of cancer.
一种微管相关蛋白（MAP）在多种类型的人类癌症中过度表达。然而，其在人类胃癌（GC）中的表达及关键作用在很大程度上仍不清楚。在此，我们进行了分子、细胞和临床分析，以评估其与GC的功能联系。FAM83D在胃肿瘤中表达升高，且其表达与淋巴结转移和TNM分期密切相关。此外，在GC细胞系中过表达FAM83D可增强细胞增殖、细胞周期进程、迁移、侵袭以及肿瘤生长和转移扩散。此外，FAM83D呈现出与细胞周期密切相关的表达。敲低FAM83D可抑制GC细胞中微管的重新生长。FAM83D与HMMR、TPX2以及有丝分裂进程的一组驱动因子AURKA共同免疫沉淀。综上所述，我们的结果表明FAM83D是人类胃癌发展中的一个重要参与者，并且是癌症治疗的一个潜在治疗靶点。
