Zhao Yuxiao, Wang Ziyi, Lao Wenwen, Kuang Ping, Jiang Nan, Yin Tao, Lin Weide, Zhu Hongyan, Ji Yonghua
a School of Life Science , Shanghai University , Shanghai , China.
Neurol Res. 2018 Jan;40(1):45-52. doi: 10.1080/01616412.2017.1390932. Epub 2017 Oct 31.
Epilepsy is a chronic neurological disorder affecting approximately 0.5-2% of the population worldwide. Gentamicin (GM) is an aminoglycoside antibiotic used to treat several types of bacterial infections. We investigate whether the administration of GM can reduce seizures in temporal lobe epilepsy (TLE).
The animal model of temporal lobe epilepsy (TLE) was established by kainic acid (KA) intrahippocampal injection. Behavioral test and Electroencephalography (EEG) recordings were performed to detect the effects of GM on the seizures triggered by KA injection in rats. Furthermore, immunofluorescence was used to investigate the influence of GM on the c-fos expression in the hippocampus.
Here we found that the intracerebroventricular administration of GM is able to prevent the seizures induced by intrahippocampal kainic acid (KA) injection. Behaviorally, the latent period to the first seizure was significantly prolonged by GM. GM can totally abolish the occurrence of stage IV or V seizures and prominently reduce the total seizure duration. Electrographic recording showed that the latent period to the first seizure, the number and duration of high-amplitude, high-frequency discharges were remarkably reduced by GM. Additionally, the expression of c-fos was significantly decreased in the ipsilateral hippocampus of KA-injected rats treated with GM compared with KA-injected rats treated with saline.
These findings could promote the understanding of the pharmacological effects of GM, enriching the application of gentamicin in clinical practice.
癫痫是一种慢性神经疾病，影响着全球约0.5%-2%的人口。庆大霉素（GM）是一种氨基糖苷类抗生素，用于治疗多种类型的细菌感染。我们研究GM的给药是否能减少颞叶癫痫（TLE）的发作。
通过海马内注射海藻酸（KA）建立颞叶癫痫（TLE）动物模型。进行行为测试和脑电图（EEG）记录，以检测GM对KA注射诱发大鼠癫痫发作的影响。此外，采用免疫荧光法研究GM对海马中c-fos表达的影响。
我们发现脑室内注射GM能够预防海马内注射海藻酸（KA）诱发的癫痫发作。行为学上，GM显著延长了首次癫痫发作的潜伏期。GM可完全消除IV期或V期癫痫发作的发生，并显著缩短癫痫发作的总时长。脑电图记录显示，GM显著缩短了首次癫痫发作的潜伏期、高幅高频放电的次数和时长。此外，与注射生理盐水的KA大鼠相比，注射GM的KA大鼠同侧海马中c-fos的表达显著降低。
这些发现有助于增进对GM药理作用的理解，丰富庆大霉素在临床实践中的应用。
