The Ritchie Centre, Hudson Institute of Medical Research and Department of Obstetrics and Gynaecology, Monash University, Melbourne, Australia.
The Ritchie Centre, Hudson Institute of Medical Research, Biotechnology Research Laboratories, Department of Physiology, Monash University, Melbourne, Australia.
Sci Rep. 2017 Oct 31;7(1):14704. doi: 10.1038/s41598-017-13113-9.
Mechanical ventilation of preterm neonates causes lung inflammation and injury, with potential life-long consequences. Inert 50-nm polystyrene nanoparticles (PS50G) reduce allergic inflammation in the lungs of adult mice. We aimed to confirm the anti-inflammatory effects of PS50G in a sheep asthma model, and investigate the effects of prophylactic administration of PS50G on ventilation-induced lung injury (VILI) in preterm lambs. We assessed lung inflammatory cell infiltration, with and without PS50G, after airway allergen challenge in ewes sensitised to house dust mite. Preterm lambs (0.83 gestation) were delivered by caesarean section for immediate tissue collection (n = 5) or ventilation either with (n = 6) or without (n = 5) prophylactic intra-tracheal administration of PS50G nanoparticles (3% in 2 ml). Ventilation was continued for a total of 2 h before tissue collection for histological and biomolecular assessment of lung injury and inflammation. In ewes with experimental asthma, PS50G decreased eosinophilic infiltration of the lungs. Ventilated preterm lambs showed molecular and histological signs of lung injury and inflammation, which were exacerbated in lambs that received PSG50G. PS50G treatment decreased established inflammation in the lungs of asthmatic sheep. However, prophylactic administration of PSG50 exacerbated ventilation-induced lung injury and lung inflammation in preterm lambs.
机械通气会导致早产儿肺部炎症和损伤，可能会产生终身影响。惰性 50nm 聚苯乙烯纳米颗粒（PS50G）可减少成年小鼠肺部的过敏炎症。我们旨在通过绵羊哮喘模型确认 PS50G 的抗炎作用，并研究 PS50G 预防性给药对早产儿羊 VILI 的影响。我们评估了在对屋尘螨致敏的母羊中气道过敏原挑战后，有和没有 PS50G 时肺部炎症细胞的浸润。通过剖宫产分娩早产儿（0.83 孕龄），立即进行组织采集（n=5）或通气，无论是否预防性给予 PS50G 纳米颗粒（3%，2ml）（n=6）或（n=5）。通气持续 2 小时，然后收集组织，进行肺损伤和炎症的组织学和生物分子评估。在患有实验性哮喘的母羊中，PS50G 减少了肺部的嗜酸性粒细胞浸润。通气的早产儿表现出分子和组织学上的肺损伤和炎症迹象，而在接受 PSG50G 的羔羊中，这些迹象更加严重。PS50G 治疗可减少哮喘羊肺部的炎症。然而，PSG50 的预防性给药加剧了早产儿羊通气诱导的肺损伤和肺部炎症。
