School of Energy and Environment, City University of Hong Kong, Hong Kong.
Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, People's Republic of China.
Bioresour Technol. 2018 Feb;249:612-619. doi: 10.1016/j.biortech.2017.10.011. Epub 2017 Oct 12.
In situ fibrous bed bioreactor (isFBB) for efficient succinic acid (SA) production by Yarrowia lipolytica was firstly developed in our former study. In this study, agricultural residues including wheat straw, corn stalk and sugarcane bagasse were investigated for the improvement of isFBB, and sugarcane bagasse was demonstrated to be the best immobilization material. With crude glycerol as the sole carbon source, optimization for isFBB batch fermentation was carried out. Under the optimal conditions of 20g sugarcane bagasse as immobilization material, 120gL crude glycerol as carbon source and 4Lmin of aeration rate, the resultant SA concentration was 53.6gL with an average productivity of 1.45gLh and a SA yield of 0.45gg. By feeding crude glycerol, SA titer up to 209.7gL was obtained from fed batch fermentation, which was the highest value that ever reported.
在我们之前的研究中，首次开发了用于高效生产琥珀酸（SA）的原位纤维床生物反应器（isFBB）。在这项研究中，我们考察了包括小麦秸秆、玉米秸秆和甘蔗渣在内的农业废弃物，以改进 isFBB，结果表明甘蔗渣是最好的固定化材料。以粗甘油为唯一碳源，对 isFBB 分批发酵进行了优化。在最佳条件下，使用 20g 甘蔗渣作为固定化材料、120g/L 粗甘油作为碳源和 4L/min 的通气速率，最终 SA 浓度为 53.6g/L，平均产率为 1.45g/Lh，SA 得率为 0.45g/g。通过补料，从分批补料发酵中获得了高达 209.7g/L 的 SA 浓度，这是迄今为止报道的最高值。
