College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China; Key Laboratory of Marine Environmental and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
Bioresour Technol. 2018 Feb;249:694-703. doi: 10.1016/j.biortech.2017.10.067. Epub 2017 Oct 20.
To gain a better understanding of the influence on two-stage anaerobic digestion of waste sludge with thermophilic bacteria (TB) and alkyl polyglucose (APG) pretreatment, changing of soluble chemical oxygen demand (SCOD), carbohydrate and protein in extracellular polymeric substances (EPS) and dissolved organic matters (DOM) were analyzed. The excitation-emission matrix (EEM) with fluorescence regional integration (FRI) was also used to investigate compositional and structural characteristics of DOM. The highest hydrogen and methane yield of TB pretreated sludge was 12.2 ml/g VS (volatile suspended solid) and 124.7 ml/g VS, and that of APG pretreated sludge was 28.3 ml/g VS and 19.9 ml/g VS. The VS removal of TB pretreated sludge (36.7%) was higher than APG pretreated sludge (27.1%) in the two-stage anaerobic digestion. The APG pretreatment could inhibit the activity of methanogens and the substrate (such as volatile fatty acids (VFAs), protein and soluble microbial materials) was accumulated compared with TB pretreatment.
为了更好地了解嗜热菌（TB）和烷基多糖苷（APG）预处理对两段式厌氧消化的影响，分析了细胞外聚合物物质（EPS）和溶解有机物质（DOM）中溶解性化学需氧量（SCOD）、碳水化合物和蛋白质的变化。还采用激发-发射矩阵（EEM）与荧光区域积分（FRI）来研究 DOM 的组成和结构特征。TB 预处理污泥的最大氢气和甲烷产率分别为 12.2 ml/gVS（挥发性悬浮固体）和 124.7 ml/gVS，APG 预处理污泥的最大氢气和甲烷产率分别为 28.3 ml/gVS 和 19.9 ml/gVS。在两段式厌氧消化中，TB 预处理污泥的 VS 去除率（36.7%）高于 APG 预处理污泥（27.1%）。与 TB 预处理相比，APG 预处理会抑制产甲烷菌的活性，导致底物（如挥发性脂肪酸（VFAs）、蛋白质和可溶性微生物物质）积累。
