Department of Pathology, Tianjin First Center Hospital, Tianjin, 300192, China.
Department of Gastric Cancer, Tianjin Cancer Institute & Hospital, Tianjin, 300060, China.
Biomed Pharmacother. 2018 Jan;97:450-457. doi: 10.1016/j.biopha.2017.10.066. Epub 2017 Nov 6.
Long non-coding RNAs (lncRNAs) play important roles on tumor development and progression in gastric cancer (GC). However, the biological function and regulatory mechanisms of LINC01296 in GC still remain unknown. The objective of this study is to investigate the clinical significance and pathological roles of LINC01296 in GC. Results showed that LINC01296 was up-regulated in GC tissue and correlated with poor prognosis. In vitro, LINC01296 knockdown was up-regulated in GC cells and LINC01296 knockdown suppressed GC cells proliferation, migration and invasion, and promoted apoptosis. In vivo xenograft assays, results showed LINC01296 knockdown significantly inhibited GC tumor growth. Bioinformatics analysis revealed that LINC01296 sponged miR-122, which was proved to target MMP-9. Western blot and RT-PCR showed that LINC01296 was positively correlated with MMP-9 expression, while miR-122 was negatively correlated to it. Overall, results indicate that LINC01296 acts as oncogenic lncRNA in GC carcinogenesis, suggesting the LINC01296/miR-122/MMP-9 regulatory pathway in GC tumorigenesis.
长链非编码 RNA（lncRNA）在胃癌（GC）的肿瘤发生和发展中发挥重要作用。然而，LINC01296 在 GC 中的生物学功能和调控机制仍不清楚。本研究旨在探讨 LINC01296 在 GC 中的临床意义和病理作用。结果表明，LINC01296 在 GC 组织中上调，并与预后不良相关。在体外，LINC01296 敲低可上调 GC 细胞中的 LINC01296，LINC01296 敲低可抑制 GC 细胞增殖、迁移和侵袭，并促进细胞凋亡。体内异种移植实验结果表明，LINC01296 敲低可显著抑制 GC 肿瘤生长。生物信息学分析表明，LINC01296 可吸附 miR-122，而 miR-122 被证明靶向 MMP-9。Western blot 和 RT-PCR 结果表明，LINC01296 与 MMP-9 表达呈正相关，而 miR-122 与之呈负相关。总之，结果表明 LINC01296 在 GC 癌发生中作为致癌 lncRNA 发挥作用，提示 LINC01296/miR-122/MMP-9 调控通路在 GC 肿瘤发生中起作用。
