Taft Robert
Cold Spring Harb Protoc. 2017 Nov 1;2017(11):pdb.prot094490. doi: 10.1101/pdb.prot094490.
Significant progress has been made in sperm cryopreservation. It is now possible to achieve more consistent fertilization rates using in vitro fertilization (IVF) with cryorecovered sperm. Including the antioxidant monothioglycerol (MTG) in the cryoprotectant may increase the fertilization rate. This protocol has been successfully used at The Jackson Laboratory since 2007 to cryopreserve >5000 strains on many genetic backgrounds. Although it is possible to recover motile sperm from 2-yr-old mice, sperm quality often begins to decline by 6 mo of age. In practice, sperm from males 10-16 wk of age has yielded the best results. This protocol is relatively easy to implement because it uses a simple freezing apparatus and a cryopreservation medium.
精子冷冻保存已取得显著进展。现在，使用经冷冻复苏的精子进行体外受精（IVF）能够实现更稳定的受精率。在冷冻保护剂中加入抗氧化剂单硫甘油（MTG）可能会提高受精率。自2007年以来，该方案已在杰克逊实验室成功用于冷冻保存5000多个不同遗传背景的品系。虽然有可能从2岁小鼠中复苏出有活力的精子，但精子质量通常在6月龄时就开始下降。实际上，10 - 16周龄雄性小鼠的精子产生的效果最佳。该方案相对容易实施，因为它使用了简单的冷冻设备和冷冻保存培养基。
