Department of Environmental Management and Control, School of Health Sciences, University of Occupational and Environmental Health.
Department of Occupational Toxicology, University of Occupational and Environmental Health.
J Occup Health. 2018 Jan 25;60(1):74-79. doi: 10.1539/joh.17-0009-BR. Epub 2017 Nov 1.
Neurotoxicity of 1-bromopropane (1-BP) has been reported in occupational exposure, but whether the chemical exerts developmental neurotoxicity is unknown. We studied the effects of prenatal 1-BP exposure on neuronal excitability in rat offspring.
We exposed dams to 1-BP (700 ppm, 6 h a day for 20 days) and examined hippocampal slices obtained from the male offspring at 2, 5, 8, and 13 weeks of age. We measured the stimulation/response (S/R) relationship and paired-pulse ratios (PPRs) of the population spike (PS) at the interpulse intervals (IPIs) of 5 and 10 ms in the CA1 subfield.
Prenatal 1-BP exposure enhanced S/R relationships of PS at 2 weeks of age; however, the enhancement diminished at 5 weeks of age until it reached control levels. Prenatal 1-BP exposure decreased PPRs of PS at 2 weeks of age. After sexual maturation, however, the PPRs of PS increased at 5-ms IPI in rats aged 8 and 13 weeks.
Our findings indicate that prenatal 1-BP exposure in dams can cause delayed adverse effects on excitability of pyramidal cells in the hippocampal CA1 subfield of offspring.
1-溴丙烷（1-BP）在职业暴露中已被报道具有神经毒性，但该化学物质是否具有发育神经毒性尚不清楚。我们研究了产前 1-BP 暴露对大鼠后代神经元兴奋性的影响。
我们使孕鼠暴露于 1-BP（700 ppm，每天 6 小时，共 20 天），并检测雄性后代在 2、5、8 和 13 周龄时获得的海马脑片。我们测量了 CA1 亚区在 5 和 10 ms 内的两个脉冲间隔（IPI）时的群体峰（PS）的刺激/反应（S/R）关系和成对脉冲比（PPR）。
产前 1-BP 暴露增强了 2 周龄时 PS 的 S/R 关系；然而，这种增强在 5 周龄时减弱，直到达到对照水平。产前 1-BP 暴露降低了 2 周龄时 PS 的 PPR。然而，在性成熟后，8 周和 13 周龄大鼠的 5 ms IPI 时 PS 的 PPR 增加。
我们的研究结果表明，母体产前 1-BP 暴露可导致后代海马 CA1 亚区锥体神经元兴奋性出现延迟性不良反应。
