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来自嗜热绿菌的蝶啶糖基转移酶：结晶与X射线分析。

Pteridine glycosyltransferase from Chlorobium tepidum: crystallization and X-ray analysis.

作者信息

Killivalavan Asaithambi, Park Young Shik, Lee Kon Ho

机构信息

Department of Convergence Biomedical Sciences, Graduate School, Gyeongsang National University, Jinju 660-751, Republic of Korea.

School of Biological Sciences, Inje University, Kimhae 621-749, Republic of Korea.

出版信息

Acta Crystallogr F Struct Biol Commun. 2017 Nov 1;73(Pt 11):629-634. doi: 10.1107/S2053230X17015515. Epub 2017 Oct 30.

DOI:10.1107/S2053230X17015515
PMID:29095157
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5683033/
Abstract

The pteridine glycosyltransferase (PGT) found in Chlorobium tepidum (CtPGT) catalyzes the conversion of L-threo-tetrahydrobiopterin to 1-O-(L-threo-biopterin-2'-yl)-β-N-acetylglucosamine using UDP-N-acetylglucosamine. The gene for CtPGT was cloned, and selenomethionine-derivatized protein was overexpressed and purified using various chromatographic techniques. The protein was crystallized by the hanging-drop vapour-diffusion method using 0.24 M triammonium citrate pH 7.0, 14%(w/v) PEG 3350 as a reservoir solution. Multiple-wavelength anomalous diffraction data were collected to 2.15 Å resolution from a single CtPGT crystal. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a = 189.61, b = 79.98, c = 105.92 Å, β = 120.5°.

摘要

在嗜热绿菌（CtPGT）中发现的蝶啶糖基转移酶（PGT）利用UDP-N-乙酰葡糖胺催化L-苏式四氢生物蝶呤转化为1-O-（L-苏式生物蝶呤-2'-基）-β-N-乙酰葡糖胺。克隆了CtPGT的基因，并用各种色谱技术对硒代蛋氨酸衍生化的蛋白质进行了过表达和纯化。使用0.24 M柠檬酸三铵pH 7.0、14%（w/v）PEG 3350作为储液，通过悬滴气相扩散法使蛋白质结晶。从单个CtPGT晶体收集了多波长反常衍射数据，分辨率达到2.15 Å。该晶体属于单斜空间群C2，晶胞参数为a = 189.61、b = 79.98、c = 105.92 Å，β = 120.5°。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17b6/5683033/4dde9ea251d3/f-73-00629-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17b6/5683033/d4fb0818153a/f-73-00629-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17b6/5683033/23a855bb13db/f-73-00629-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17b6/5683033/a185dd56f521/f-73-00629-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17b6/5683033/4dde9ea251d3/f-73-00629-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17b6/5683033/d4fb0818153a/f-73-00629-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17b6/5683033/23a855bb13db/f-73-00629-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17b6/5683033/a185dd56f521/f-73-00629-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/17b6/5683033/4dde9ea251d3/f-73-00629-fig4.jpg

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