1 Department of Bioengineering, University of Texas at Dallas, Richardson, TX, USA.
SLAS Technol. 2018 Feb;23(1):5-15. doi: 10.1177/2472630317738700. Epub 2017 Nov 2.
Point-of-care (POC) testing has revolutionized diagnostic healthcare, bringing medical results directly and immediately to the patient. With faster diagnostics, more immediate clinical management decisions can be made. POC tests most often use a dipstick or swab format to detect the presence of a pathogen, disease, or other relevant biomarker. In these formats, the POC tests eliminate the need for complex lab equipment and trained personnel to collect, process, and analyze sample data for simple diagnostics. However, these tests cannot satisfy all clinical needs, because accurate quantitative results are needed. The present study serves as a template for designing a nonfaradaic electrochemical biosensor toward quantitative POC diagnostics. We focus on investigating the most important parameters when constructing a nonfaradaic biosensor through both mathematical modeling and electrochemical measurements. Furthermore, we demonstrate quantitative affinity biosensing of a model protein toward developing a POC device.
即时检测（POC）技术革新了诊断医疗，将医疗结果直接、即时地呈现给患者。通过更快的诊断，能够更快地做出更直接的临床管理决策。POC 检测通常采用试条或拭子的形式来检测病原体、疾病或其他相关生物标志物的存在。在这些形式中，POC 检测消除了对复杂实验室设备和训练有素的人员的需求，无需他们收集、处理和分析样本数据进行简单诊断。然而，这些检测并不能满足所有临床需求，因为需要准确的定量结果。本研究为设计用于定量即时检测诊断的非法拉第电化学生物传感器提供了模板。我们通过数学建模和电化学测量，重点研究了构建非法拉第生物传感器时最重要的参数。此外，我们还展示了针对模型蛋白的定量亲和生物传感，以开发即时检测设备。
