Assistant Professor, Department of Dentistry, Health Science Centre, Federal University of Santa Catarina, Florianópolis, Brazil.
Scientific Laboratory Technician, Department of Oral Biology, School of Dentistry, University of São Paulo, Bauru, Brazil.
Implant Dent. 2017 Dec;26(6):875-881. doi: 10.1097/ID.0000000000000694.
To evaluate if an inorganic graft applied before implant insertion interferes with osseointegration.
The bilateral mandibular incisors of 12 rabbits were extracted. One of the sockets was randomly filled with an inorganic xenogenic bone graft, whereas the remaining socket was allowed to heal naturally and served as a control. After 60 days, titanium implants were inserted into healing areas. The animals were killed 60 days after. Bone depositions were marked with fluorochrome oxytetracycline, alizarin, and calcein and evaluated using confocal laser scanning microscopy. Bone-to-implant contact (BIC) and bone area (BA) within the limits of the implant threads were analyzed. Data were compared statically by paired t tests, one-way ANOVA, and Bonferroni post hoc tests (α = 0.05).
No differences between the control and experimental groups in bone deposition for each marker, in either the BIC or BA analysis were observed. The bone deposition marked by alizarin (14-21 days) was the highest, followed by oxytetracycline (0 and 7 days) and calcein (45 and 52 days) in both groups (P < 0.05).
The bone healing or the course of osseointegration was not impaired by the use of an inorganic xenogenic graft before insertion of a titanium implant.
评估在植入物插入前应用无机移植物是否会干扰骨整合。
将 12 只兔子的双侧下颌切牙拔除。其中一个牙槽窝随机填充无机异种骨移植物，而其余牙槽窝自然愈合作为对照。60 天后，将钛植入物插入愈合区域。动物在植入后 60 天被处死。用荧光四环素、茜素红和钙黄绿素标记骨沉积，并使用共聚焦激光扫描显微镜进行评估。分析了种植体螺纹范围内的骨-种植体接触（BIC）和骨面积（BA）。数据通过配对 t 检验、单因素方差分析和 Bonferroni 事后检验（α=0.05）进行静态比较。
在每个标记物的 BIC 或 BA 分析中，对照组和实验组之间的骨沉积没有差异。两组中，茜素红（14-21 天）标记的骨沉积最高，其次是四环素（0 和 7 天）和钙黄绿素（45 和 52 天）（P<0.05）。
在插入钛植入物之前使用无机异种骨移植物不会损害骨愈合或骨整合过程。
