Lee Chung-Gi, Choi Jae-Hwan, Park Chanhun, Wang Nien-Hwa Linda, Mun Sungyong
Department of Chemical Engineering, Hanyang University, Haengdang-dong, Seongdong-gu, Seoul, 04763, South Korea.
School of Chemical Engineering, 480 Stadium Mall Drive, Purdue University, West Lafayette, IN 47907-2100, USA.
J Chromatogr A. 2017 Dec 8;1527:80-90. doi: 10.1016/j.chroma.2017.10.067. Epub 2017 Oct 28.
The feasibility of a simulated moving bed (SMB) technology for the continuous separation of high-purity xylobiose (X2) from the output of a β-xylosidase X1→X2 reaction has recently been confirmed. To ensure high economical efficiency of the X2 production method based on the use of xylose (X1) as a starting material, it is essential to accomplish the comprehensive optimization of the X2-separation SMB process in such a way that its X2 productivity can be maximized while maintaining the X2 product concentration from the SMB as high as possible in consideration of a subsequent lyophilization step. To address this issue, a suitable SMB optimization tool for the aforementioned task was prepared based on standing wave design theory. The prepared tool was then used to optimize the SMB operation parameters, column configuration, total column number, adsorbent particle size, and X2 yield while meeting the constraints on X2 purity, X2 product concentration, and pressure drop. The results showed that the use of a larger particle size caused the productivity to be limited by the constraint on X2 product concentration, and a maximum productivity was attained by choosing the particle size such that the effect of the X2-concentration limiting factor could be balanced with that of pressure-drop limiting factor. If the target level of X2 product concentration was elevated, higher productivity could be achieved by decreasing particle size, raising the level of X2 yield, and increasing the column number in the zones containing the front and rear of X2 solute band.
最近已证实，模拟移动床（SMB）技术可用于从β-木糖苷酶X1→X2反应的产物中连续分离高纯度木二糖（X2）。为确保以木糖（X1）为起始原料的X2生产方法具有较高的经济效率，必须对X2分离SMB工艺进行全面优化，以便在考虑后续冻干步骤的情况下，在尽可能保持SMB中X2产物浓度高的同时，使X2生产率最大化。为解决此问题，基于驻波设计理论制备了适用于上述任务的SMB优化工具。然后使用该工具在满足X2纯度、X2产物浓度和压降等约束条件下，对SMB操作参数、柱配置、总柱数、吸附剂粒径和X2产率进行优化。结果表明，使用较大粒径会使生产率受到X2产物浓度约束的限制，通过选择粒径使X2浓度限制因素的影响与压降限制因素的影响达到平衡，可实现最大生产率。如果提高X2产物浓度的目标水平，则通过减小粒径、提高X2产率水平以及增加包含X2溶质带前后区域的柱数，可实现更高的生产率。
