Yao Xiaolei, Ei-Samahy M A, Feng Xu, Zhang Tinging, Li Fengzhe, Zhang Guomin, Pang Jing, Nie Haitao, Wang Feng
Jiangsu Engineering Technology Research Center of Mutton Sheep and Goat Industry, Nanjing Agricultural University, Nanjing 210095, PR China.
Jiangsu Engineering Technology Research Center of Mutton Sheep and Goat Industry, Nanjing Agricultural University, Nanjing 210095, PR China.
Anim Reprod Sci. 2017 Dec;187:159-166. doi: 10.1016/j.anireprosci.2017.10.020. Epub 2017 Oct 27.
The polypeptide N-acetylgalactosaminyltransferase-like protein 5 (GALNTL5) is involved in male fertility; however, its involvement in the reproduction and fertility of females remains unclear. Therefore, the present study aimed to examine the presence of GALNTL5 in the reproductive organs of ewes during the estrus period and to investigate its expression in cauda epididymal and ejaculated sperm. Results showed that GALNTL5 mRNA and protein were present in some reproductive organs of ewes during the estrus period. The highest levels of GALNTL5 mRNA and protein occurred in the uterine horn and oviductal ampulla and the lowest in the uterine cervix and oviductal infundibulum. Immunohistochemical analysis revealed that GALNTL5 protein was mainly located in luminal and glandular epithelial cells of the uterus and oviduct, and in the theca and granulosa cells of the ovary. GALNTL5 gene expression was significantly higher in ejaculated sperm than in cauda epididymal sperm. The amount of GALNTL5 protein in seminal plasma was significantly higher than in ejaculated sperm. Additionally, GALNTL5 was strongly localized in the mid-piece and head of ejaculated sperm, and in the head-tail coupling apparatus and acrosome of cauda epididymal sperm. This is the first evidence that GALNTL5 might play an important role in a range of reproductive functions as well as in sperm motility and capacitation. Further studies are required to evaluate the function of GALNTL5 in reproduction.
多肽N - 乙酰半乳糖胺基转移酶样蛋白5（GALNTL5）与雄性生育力有关；然而，其在雌性生殖和生育中的作用仍不清楚。因此，本研究旨在检测发情期母羊生殖器官中GALNTL5的存在情况，并研究其在附睾尾精子和射出精子中的表达。结果表明，发情期母羊的一些生殖器官中存在GALNTL5 mRNA和蛋白质。GALNTL5 mRNA和蛋白质水平在子宫角和输卵管壶腹部最高，在子宫颈和输卵管漏斗部最低。免疫组织化学分析显示，GALNTL5蛋白主要位于子宫和输卵管的管腔和腺上皮细胞，以及卵巢的膜细胞和颗粒细胞中。GALNTL5基因在射出精子中的表达明显高于附睾尾精子。精浆中GALNTL5蛋白的含量明显高于射出精子。此外，GALNTL5在射出精子的中段和头部以及附睾尾精子的头尾连接装置和顶体中强烈定位。这是GALNTL5可能在一系列生殖功能以及精子活力和获能中发挥重要作用的首个证据。需要进一步研究来评估GALNTL5在生殖中的功能。
