Ma Bin, Hua Kexin, Zhou Shanshan, Zhou Hufeng, Chen Yushan, Luo Rui, Bi Dingren, Zhou Rui, He Qigai, Jin Hui
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Dev Comp Immunol. 2018 Feb;79:158-165. doi: 10.1016/j.dci.2017.10.021. Epub 2017 Oct 31.
Haemophilus parasuis, an important swine pathogen, was recently proven able to invade into endothelial or epithelial cell in vitro. NOD1/2 are specialized NLRs that participate in the recognition of pathogens able to invade intracellularly and therefore, we assessed that the contribution of NOD1/2 to inflammation responses during H. parasuis infection. We observed that H. parasuis infection enhanced NOD2 expression and RIP2 phosphorylation in porcine kidney 15 cells. Our results also showed that knock down of NOD1/2 or RIP2 expression respectively significantly decreased H. parasuis-induced NF-κB activity, while the phosphorylation level of p38, JNK or ERK was not changed. Moreover, real-time PCR result showed that NOD1, NOD2 or RIP2 was involved in the expression of CCL4, CCL5 and IL-8. Inhibition of NOD1 and NOD2 significantly reduced CCL5 promoter activity, even in a more effective way compared with inhibition of TLR.
副猪嗜血杆菌是一种重要的猪病原体，最近被证实能够在体外侵入内皮细胞或上皮细胞。NOD1/2是专门的NLRs，参与识别能够侵入细胞内的病原体，因此，我们评估了NOD1/2在副猪嗜血杆菌感染期间对炎症反应的作用。我们观察到副猪嗜血杆菌感染增强了猪肾15细胞中NOD2的表达和RIP2的磷酸化。我们的结果还表明，分别敲低NOD1/2或RIP2的表达显著降低了副猪嗜血杆菌诱导的NF-κB活性，而p38、JNK或ERK的磷酸化水平没有改变。此外，实时PCR结果表明，NOD1、NOD2或RIP2参与了CCL4、CCL5和IL-8的表达。抑制NOD1和NOD2显著降低了CCL5启动子活性，甚至比抑制TLR更有效。
