Department of Biology, University of Ottawa, Ontario, K1N 6N5, Canada.
Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida Genetics Institute, Interdisciplinary Program in Biomedical Sciences Neuroscience, College of Veterinary Medicine, University of Florida, Gainesville, FL, 32611, USA.
Sci Rep. 2017 Nov 2;7(1):14930. doi: 10.1038/s41598-017-14930-8.
Radial glial cells (RGCs) are the most abundant macroglia in the teleost brain and have established roles in neurogenesis and neurosteroidogenesis; however, their transcriptome remains uncharacterized, which limits functional understanding of this important cell type. Using cultured goldfish RGCs, RNA sequencing and de novo transcriptome assembly were performed, generating the first reference transcriptome for fish RGCs with 17,620 unique genes identified. These data revealed that RGCs express a diverse repertoire of receptors and signaling molecules, suggesting that RGCs may respond to and synthesize an array of hormones, peptides, cytokines, and growth factors. Building upon neuroanatomical data and studies investigating direct neuronal regulation of RGC physiology, differential gene expression analysis was conducted to identify transcriptional networks that are responsive to the conserved secretogranin II-derived neuropeptide secretoneurin A (SNa). Pathway analysis of the transcriptome indicated that cellular processes related to the central nervous system (e.g., neurogenesis, synaptic plasticity, glial cell development) and immune functions (e.g., immune system activation, leukocyte function, macrophage response) were preferentially modulated by SNa. These data reveal an array of new functions that are proposed to be critical to neuronal-glial interactions through the mediator SNa.
放射状胶质细胞（RGCs）是硬骨鱼类脑中最丰富的大胶质细胞，它们在神经发生和神经甾体生成中具有重要作用；然而，它们的转录组尚未被描述，这限制了对这种重要细胞类型的功能理解。使用培养的金鱼 RGCs，进行了 RNA 测序和从头转录组组装，生成了第一个鱼类 RGCs 的参考转录组，鉴定出 17620 个独特基因。这些数据表明，RGCs 表达了一系列多样化的受体和信号分子，表明 RGCs 可能对一系列激素、肽、细胞因子和生长因子做出反应并合成它们。在神经解剖学数据和研究直接调节 RGC 生理学的神经元的基础上，进行了差异基因表达分析，以鉴定对保守的分泌颗粒 II 衍生的神经肽分泌素 A（SNa）有反应的转录网络。对转录组的途径分析表明，与中枢神经系统相关的细胞过程（例如，神经发生、突触可塑性、神经胶质细胞发育）和免疫功能（例如，免疫系统激活、白细胞功能、巨噬细胞反应）优先被 SNa 调节。这些数据揭示了一系列新的功能，这些功能被认为通过中介 SNa 对神经元-神经胶质相互作用至关重要。
