Bothra Shilpa, Babu Lavanya Thilak, Paira Priyankar, Ashok Kumar S K, Kumar Rajender, Sahoo Suban K
Department of Applied Chemistry, SV National Institute of Technology (SVNIT), Surat, 395007, India.
Pharmaceutical Chemistry Division, School of Advanced Sciences, VIT University, Vellore, 632014, India.
Anal Bioanal Chem. 2018 Jan;410(1):201-210. doi: 10.1007/s00216-017-0710-2. Epub 2017 Nov 2.
This communication focusses on the synthesis of red fluorescent lysozyme cocooned gold nanoclusters (Lyso-AuNCs) that have been successfully applied for the selective and specific recognition of the vitamin B cofactor pyridoxal-5'-phosphate (PLP). The red fluorescence of Lyso-AuNCs showed remarkable color change to yellow upon conjugation with PLP due to the formation of a Schiff base between the free -NH present in the lysozyme and the -CHO group of PLP. The developed PLP conjugated Lyso-AuNCs (PLP_Lyso-AuNCs) was applied for the selective turn-on recognition of Zn ions in aqueous medium. The yellow fluorescence of PLP_Lyso-AuNCs exhibited significant enhancement at 475 nm in the presence of Zn producing bluish-green fluorescence attributed to the complexation-induced aggregation of nanoclusters. The nanoprobe exhibits nanomolar limit of detection for Zn ions (39.2 nM) and the practicality of the nanoprobe was validated in various environmental water samples and biological plasma, urine, and beetroot extract, with fairly good recovery percent. Also, the system was successfully implemented for the intracellular detection and monitoring of Zn in live HeLa cells. Graphical abstract Applications of red emitting lysozyme cocooned gold nanoclusters (Lyso-AuNCs) for the selective recognition of the vitamin B cofactor pyridoxal-5'-phosphate (PLP) and the conjugated nano-assembly PLP_Lyso-AuNCs for turn-on detection of Zn ions in various environmental and biological samples.
本通讯聚焦于红色荧光溶菌酶包裹的金纳米簇（Lyso-AuNCs）的合成，该纳米簇已成功应用于维生素B辅因子磷酸吡哆醛（PLP）的选择性和特异性识别。Lyso-AuNCs的红色荧光在与PLP结合后由于溶菌酶中存在的游离-NH与PLP的-CHO基团之间形成席夫碱而显著变为黄色。所开发的PLP共轭Lyso-AuNCs（PLP_Lyso-AuNCs）用于在水性介质中选择性开启识别锌离子。PLP_Lyso-AuNCs的黄色荧光在锌存在下于475nm处显著增强，产生蓝绿色荧光，这归因于纳米簇的络合诱导聚集。该纳米探针显示出对锌离子的纳摩尔检测限（39.2 nM），并且该纳米探针的实用性在各种环境水样以及生物血浆、尿液和甜菜根提取物中得到验证，回收率相当高。此外，该系统成功用于活HeLa细胞中锌的细胞内检测和监测。图形摘要：红色发射的溶菌酶包裹的金纳米簇（Lyso-AuNCs）用于选择性识别维生素B辅因子磷酸吡哆醛（PLP）以及共轭纳米组装体PLP_Lyso-AuNCs用于在各种环境和生物样品中开启检测锌离子的应用。
