Department of Biodiversity and Experimental Biology, FCEN, Institute of Biodiversity, Experimental and Applied Biology (IBBEA), CONICET-UBA, University of Buenos Aires, Ciudad Universitaria, Pab. II, C1428EGA, Buenos Aires, Argentina.
Environ Sci Pollut Res Int. 2018 Jan;25(2):1568-1575. doi: 10.1007/s11356-017-0581-2. Epub 2017 Nov 2.
Adult females of the estuarine crab Neohelice granulata were exposed to the glyphosate formulation Roundup Ultramax® during the entire 3-month pre-reproductive period. At the end of the assay, a significant higher increment of glycemia was noted at both glyphosate concentrations assayed (0.01 and 0.2 mg/L, acid equivalent). Although no differences were observed in the gonadosomatic index, a significantly higher proportion of reabsorbed vitellogenic oocyte was observed at the highest glyphosate concentration, together with a significant decrease of vitellogenin content in the ovary. In addition, some in vitro assays were carried out by co-incubating small pieces of ovary with or without the addition of Roundup; at both concentrations tested (same as those used in vivo), a decrease in the ovarian vitellogenin content was observed, whereas the ovarian protein synthesis was significantly inhibited by glyphosate at 0.2 mg/L in the Roundup formulation used.
在整个 3 个月的生殖前期，成年雌性港湾梭子蟹被暴露于草甘膦制剂农达®UltraMax 中。在试验结束时，在两种草甘膦浓度（0.01 和 0.2mg/L，酸当量）下均观察到血糖升高的显著增加。尽管性腺指数没有差异，但在最高草甘膦浓度下观察到更多的已吸收卵黄生成卵母细胞，同时卵巢中的卵黄蛋白含量显著下降。此外，通过将小块卵巢与或不与农达一起共孵育进行了一些体外试验；在测试的两种浓度下（与体内使用的相同），观察到卵巢卵黄蛋白含量下降，而在农达制剂中 0.2mg/L 的草甘膦显著抑制了卵巢蛋白的合成。
