Lohinova O, Pitko V, Sinilo N, Cherkashyna Ya, Hanzha N
Kharkiv Medical Academy of Post-graduate Education, Department of obstetrics and gynecology №1; State Institution "Ukrainian Medical Center of Obstetrics, Gynecology and Reproductology of Health Care Ministry of Ukraine", Kharkiv, Ukraine.
Georgian Med News. 2017 Oct(271):7-11.
In recent years, one of the methods of assisted reproductive technologies has become increasingly widespread, namely the ICSI procedure (ICSI - Intra Cytoplasmic Sperm Injection). This method is based on artificial introduction of the spermatozoon into the cytoplasm of the oocyte. However, the complexity of this method is that the choice of a morphologically and biochemically mature spermatozoon becomes subjective. However, not so long ago, an innovative method for selecting mature, ready-to-fertilize spermatozoa was proposed. This method was called PICSI (PICSI - Physiologic Intra Cytoplasmic Sperm Injection). It is based on the ability of mature spermatozoa to bind to hyaluronic acid. Currently, there are two methods for selection of mature spermatozoa with hyaluronic acid: polystyrene culture cups the inner surface of which are coated with microcap lagialuronate (PICSI Sperm Selection Device; Mid Atlantic Diagnostic - Origio) and a liquid medium containing hyaluronic acid (Sperm Slow; Medi Cult - Origio). We selected 60 family couples with a history of one or more unsuccessful attempts at in vitro fertilization or early reproductive loss in an anamnesis after infertility treatment. The potential impact of an abnormal parental genome on the reproductive outcome of extracorporeal fertilization cycles has not yet been fully understood. However, in the last ten years, the quality of sperm DNA is considered one of the possible reasons for the poor quality of the embryos, especially after the ICSI procedure. Sperm selection, based only on microscopic analysis, can lead to inadequate evaluation and selection of spermatozoa with chromosomal or cytoplasmic abnormalities in a visually morphologically normal cell. Studies have shown that this method of selection of mature spermatozoa, such as PICSI, is a very effective addition to the ICSI procedure in couples with predominance of the male factor of infertility. A possible reason for the effectiveness of this method is a more physiological selection of mature spermatozoa and, as a consequence, a decrease in the number of participating in the cycles of IVF spermatozoa with an anomalous genome. The use of both PICSI cups and media containing hyaluronic acid in the liquid form of SpermSlow for the selection of mature spermatozoa is equally ffective.
近年来，辅助生殖技术的一种方法越来越普及，即卵胞浆内单精子注射程序（ICSI - 卵胞浆内单精子注射）。该方法基于将精子人工注入卵母细胞的细胞质中。然而，这种方法的复杂性在于，形态学和生物化学成熟精子的选择变得主观。不过，不久前，一种选择成熟、可受精精子的创新方法被提出。这种方法被称为生理性卵胞浆内单精子注射（PICSI - Physiologic Intra Cytoplasmic Sperm Injection）。它基于成熟精子与透明质酸结合的能力。目前，有两种使用透明质酸选择成熟精子的方法：聚苯乙烯培养杯，其内表面涂有微胶囊化透明质酸（PICSI精子选择装置；大西洋中部诊断公司 - Origio）和含有透明质酸的液体培养基（Sperm Slow；Medi Cult - Origio）。我们选择了60对有过一次或多次体外受精失败或不育治疗后早期生殖损失病史的家庭夫妇。异常亲代基因组对体外受精周期生殖结果的潜在影响尚未完全了解。然而，在过去十年中，精子DNA质量被认为是胚胎质量差的可能原因之一，尤其是在ICSI程序之后。仅基于显微镜分析的精子选择可能导致对视觉形态正常细胞中具有染色体或细胞质异常的精子评估和选择不足。研究表明，对于男性不育因素占主导的夫妇，这种选择成熟精子的方法，如PICSI，是ICSI程序非常有效的补充。这种方法有效性的一个可能原因是对成熟精子进行了更生理性的选择，结果是参与体外受精周期的基因组异常精子数量减少。使用PICSI杯和含有液体形式透明质酸的SpermSlow培养基选择成熟精子同样有效。
