Sun Fenfen, Pan Wei, Gao Honglei, Qi Xiaole, Qin Liting, Wang Yongqiang, Gao Yulong, Wang Xiaomei
Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China; Experimental Teaching Center of Morphology, Xuzhou Medical University, Xuzhou 221004, Jiangsu Province, China.
Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Virology. 2018 Jan 1;513:188-194. doi: 10.1016/j.virol.2017.09.014. Epub 2017 Nov 5.
Chicken anemia virus (CAV) is a small, single-stranded DNA virus of Anelloviridae family. Its genome segments encode three proteins, VP1, VP2, and VP3. This study identified an interaction between VP2 and VP3 and mapped the interaction domains. Through the yeast two-hybrid (Y2H) system, VP2 was found to interact with VP3. The presence of the VP2-VP3 complex in CAV-infected chicken cells was confirmed by co-immunoprecipitation. Confocal microscopy showed that VP2 and VP3 were expressed in the cytoplasm in cotransfected Vero cells. In the Y2H system, the interaction domains were identified as being within the N-terminal aa 1-30 and C-terminal aa 17-60 for VP2 and the N-terminal aa 46-60 and C-terminal aa 1-7 for VP3. This study showed the interaction between VP2 and VP3 of CAV and identified multiple independent interactive domains within the two proteins. This provides novel information for investigating the biological functions of these proteins.
鸡贫血病毒（CAV）是指环病毒科的一种小型单链DNA病毒。其基因组片段编码三种蛋白质，即VP1、VP2和VP3。本研究确定了VP2和VP3之间的相互作用，并绘制了相互作用结构域。通过酵母双杂交（Y2H）系统，发现VP2与VP3相互作用。通过免疫共沉淀证实了CAV感染的鸡细胞中存在VP2-VP3复合物。共聚焦显微镜显示，VP2和VP3在共转染的Vero细胞的细胞质中表达。在Y2H系统中，VP2的相互作用结构域被确定为N端第1至30个氨基酸和C端第17至60个氨基酸，VP3的相互作用结构域被确定为N端第46至60个氨基酸和C端第1至7个氨基酸。本研究展示了CAV的VP2和VP3之间的相互作用，并确定了这两种蛋白质内多个独立的相互作用结构域。这为研究这些蛋白质的生物学功能提供了新信息。
