Flieger Jolanta, Tatarczak-Michalewska Małgorzata, Kowalska Anna, Madejska Anna, Śniegocki Tomasz, Sroka-Bartnicka Anna, Szymańska-Chargot Monika
Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4A, 20-093 Lublin, Poland.
Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4A, 20-093 Lublin, Poland.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Dec 1;1070:1-6. doi: 10.1016/j.jchromb.2017.10.021. Epub 2017 Oct 26.
The new sorbent for solid phase extraction (SPE), based on silica gel modified with a copper (II), was obtained and its application for phospholipids removal from the human plasma was tested. SPE column conditioning requirements, the volume of the plasma, the composition of the elution solvent were all established. The efficacy of the removal of phospholipids was compared for different methods such as standard protein precipitation or HybridSPE Phospholipid Ultra and HybridSPE-PPT. The sample clean-up was verified by mass spectrometry (MS) and by monitoring of chromatograms in the region between 190nm and 400nm. The Fourier Transform Infrared Spectroscopy FT-IR and confocal Raman microscopy were used to evaluating the silica gel modifications and to show the structure of lipids confined in the silica pores.
制备了一种基于用铜（II）改性硅胶的新型固相萃取（SPE）吸附剂，并测试了其用于从人血浆中去除磷脂的应用。确定了SPE柱的预处理要求、血浆体积和洗脱溶剂的组成。比较了不同方法（如标准蛋白沉淀法、HybridSPE磷脂超滤法和HybridSPE-PPT法）去除磷脂的效果。通过质谱（MS）以及监测190nm至400nm区域的色谱图对样品净化进行了验证。使用傅里叶变换红外光谱（FT-IR）和共聚焦拉曼显微镜来评估硅胶的改性情况，并展示限制在硅胶孔中的脂质结构。
