Ohnishi Takahiro, Kubo Aya, Yoshinari Tomoya, Watanabe Maiko
Division of Microbiology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya, Tokyo, 158-8501, Japan.
Parasitol Res. 2018 Jan;117(1):303-306. doi: 10.1007/s00436-017-5665-x. Epub 2017 Nov 3.
Kudoa septempunctata, a myxosporean parasite, is the causative agent of a foodborne illness associated with consumption of raw Paralichthys olivaceus (olive flounder). Because the lag phase of this illness is short (from 1 to 12 h), it is possible that an allergic response is relevant to this illness. To test whether a K. septempunctata antigen is the possible allergen, we injected a myxospore extract into BALB/c mice and measured IgE levels in serum. When the mice were injected with the myxospore extract, the total serum IgE concentration increased significantly after the second immunization as compared to the negative control. After the third immunization, total IgE concentration in the immunized mice reached 26.5 ng/ml and was almost equivalent to that of egg albumin-injected mice. Western blot analysis revealed that IgE antibodies-in serum samples that were collected from myxospore extract-injected mice-bound to at least two Kudoa proteins with molecular weight between 28 and 36 kDa. These results suggested that a K. septempunctata antigen is the allergen. Further studies are needed to clarify the contribution of allergy to the foodborne illness caused by K. septempunctata.
七斑库道虫（Kudoa septempunctata）是一种粘孢子虫寄生虫，是与食用生牙鲆（Paralichthys olivaceus，又称褐牙鲆）相关的食源性疾病的病原体。由于这种疾病的潜伏期很短（1至12小时），因此过敏反应有可能与该疾病有关。为了测试七斑库道虫抗原是否可能是过敏原，我们将粘孢子虫提取物注射到BALB/c小鼠体内，并测量血清中的IgE水平。当给小鼠注射粘孢子虫提取物时，与阴性对照相比，第二次免疫后血清总IgE浓度显著增加。第三次免疫后，免疫小鼠的总IgE浓度达到26.5 ng/ml，几乎与注射卵清蛋白的小鼠相当。蛋白质印迹分析显示，从注射粘孢子虫提取物的小鼠收集的血清样本中的IgE抗体与至少两种分子量在28至36 kDa之间的库道虫蛋白结合。这些结果表明七斑库道虫抗原是过敏原。需要进一步研究以阐明过敏对七斑库道虫引起的食源性疾病的作用。
