凤冠兰对老年Wistar大鼠慢性疲劳综合征的缓解作用。
Effect of Coelogyne cristata Lindley in alleviation of chronic fatigue syndrome in aged Wistar rats.
作者信息
Mitra Achintya, Sur Tapas Kumar, Upadhyay Sachhidananda, Bhattacharyya Dipankar, Hazra Jayram
机构信息
National Research Institute of Ayurvedic Drug Development, Kolkata Under CCRAS, Min. of AYUSH, Government of India, 4 CN Block, Sector-V, Bidhan Nagar, Kolkata, 700 091, India.
Department of Pharmacology, Institute of Post Graduate Medical Education and Research, 24 A.J.C. Bose Road, Kolkata, 700 020, India.
出版信息
J Ayurveda Integr Med. 2018 Oct-Dec;9(4):266-271. doi: 10.1016/j.jaim.2017.06.011. Epub 2017 Nov 1.
BACKGROUND
Swarna Jibanti scientifically known as Coelogyne cristata Lindley (Orchidaceae), an orchid mentioned in Ayurvedic medicine is used to promote healthy life span.
OBJECTIVE(S): The present work was planned to study the efficacy of hydro-alcoholic extract of pseudobulbs of C. cristata (CCE) to assess its role on chronic fatigue syndrome (CFS) induced behavioural and biochemical changes in aged Wistar rats compared to Panax ginseng (PG), a prototype anti-stress agent.
MATERIALS AND METHODS
CFS was induced by forced swimming for consecutive 21 days for fixed duration (15 min sessions). The criteria of CFS due to fatigue were counted using locomotor activity, depression and anxiety through automated photactometer, immobility time and plus maze activity respectively. Acute toxicity study of CCE (upto 2 g/kg, Limit test) was also performed. For CFS, animals were divided into five groups, naive control, control, CCE treated (25 mg/kg b.w., 250 mg/kg b.w.) and standard PG treated (100 mg/kg b.w.) groups. All drugs were given orally for consecutive 21 days along with CFS. After assessing behavioural parameters, all animals were sacrificed at day 21 and in vivo antioxidant potential of CCE was determined by lipid peroxides, nitrite, catalase (CAT) and superoxide dismutase (SOD) in brain tissue.
RESULTS
CCE was found to be non-toxic. CCE treated aged rats significantly improved (p < 0.001) the spontaneous locomotor movement with respect to control rats, while, decreased the mobility period or depression score. In CFS, CCE also enhanced the time spent (p < 0.001) in open arms while reducing the time spent in closed arm as compared to CFS control, indicating lowering anxiety score. Moreover, marked diminution in lipid peroxidation, nitrite and SOD level was exhibited after CCE treatment and significantly enhanced catalase level significantly (p < 0.01) with respect to CFS control. PG also showed similar actions.
CONCLUSION
The results confirmed the potential therapeutic actions of CCE against experimentally induced CFS in aged rats that might be due to its CNS mediatory antioxidant properties.
背景
Swarna Jibanti，科学名称为Coelogyne cristata Lindley（兰科），是阿育吠陀医学中提到的一种兰花，用于促进健康长寿。
目的
本研究旨在探讨Coelogyne cristata假鳞茎的水醇提取物（CCE）的功效，以评估其对老年Wistar大鼠慢性疲劳综合征（CFS）诱导的行为和生化变化的作用，并与原型抗应激剂人参（PG）进行比较。
材料与方法
通过连续21天每天固定时长（15分钟）的强迫游泳诱导CFS。分别通过自动光电计、不动时间和十字迷宫活动，利用运动活性、抑郁和焦虑来计算因疲劳导致的CFS标准。还进行了CCE的急性毒性研究（高达2 g/kg，极限试验）。对于CFS，将动物分为五组：未处理对照、对照、CCE处理组（25 mg/kg体重，250 mg/kg体重）和标准PG处理组（100 mg/kg体重）。所有药物与CFS诱导同时连续口服21天。在评估行为参数后，于第21天处死所有动物，并通过脑组织中的脂质过氧化物、亚硝酸盐、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）测定CCE的体内抗氧化潜力。
结果
发现CCE无毒。与对照大鼠相比，CCE处理的老年大鼠自发运动显著改善（p < 0.001），同时，活动期或抑郁评分降低。在CFS模型中，与CFS对照组相比，CCE还增加了在开放臂中花费的时间（p < 0.001），同时减少了在封闭臂中花费的时间，表明焦虑评分降低。此外，CCE处理后脂质过氧化、亚硝酸盐和SOD水平显著降低，过氧化氢酶水平相对于CFS对照组显著升高（p < 0.01）。PG也表现出类似作用。
结论
结果证实了CCE对老年大鼠实验性诱导的CFS具有潜在治疗作用，这可能归因于其对中枢神经系统的介导抗氧化特性。
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