Nephrology Division, Department of Medicine, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Brazil.
Experimental Neurology Division, Department of Neurology and Neurosurgery, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Brazil, Brazil.
Int J Biol Macromol. 2018 Mar;108:817-825. doi: 10.1016/j.ijbiomac.2017.10.179. Epub 2017 Nov 2.
The (pro)renin receptor [(P)RR] has been implicated as a renin/prorenin receptor, and plays a role in local renin angiotensin system activation. Our goal was to investigate whether a transgenic mouse that expresses rat tonin [TGM'(rTon)] can regulate (P)RR mRNA levels. Control (C) and TGM'(rTon) animals were subdivided into the C sham, C MI, TGM'(rTon) sham, and TGM'(rTon) MI groups. The levels of tonin, (P)RR, and renin were determined using RT-PCR mRNA. Tonin activity as determined by RIE was significantly increased in the TGM'(rTon) sham group as compared to the C sham group in the atrium (AT) and right ventricle (RV), respectively. In most mice, tonin mRNA levels were significantly reduced compared to those in the TGM'(rTon) sham group in the atria. In this structure, the (P)RR mRNA levels were statistically significantly reduced in the TGM'(rTon) sham and TGM'(rTon) MI groups compared to the control groups. However, the (P)RR mRNA values were significantly increased when we compared the TGM'(rTon) MI vs TGM'(rTon) sham groups. In the RV, the renin mRNA levels in the TGM'(rTon) sham group were significantly reduced compared to the C sham group. Tonin overexpression may act in the regulation of (P)RR mRNA levels during MI.
（前）肾素受体 [(P)RR] 已被认为是肾素/前肾素受体，在局部肾素血管紧张素系统激活中发挥作用。我们的目标是研究表达大鼠张力素 [TGM'(rTon)] 的转基因小鼠是否可以调节 (P)RR mRNA 水平。将对照 (C) 和 TGM'(rTon) 动物分为 C 假手术、C 心肌梗死、TGM'(rTon) 假手术和 TGM'(rTon) 心肌梗死组。使用 RT-PCR mRNA 测定张力素、(P)RR 和肾素的水平。与 C 假手术组相比，TGM'(rTon) 假手术组心房 (AT) 和右心室 (RV) 中的张力素活性分别通过 RIE 显著增加。在大多数小鼠中，与 TGM'(rTon) 假手术组相比，心房中的张力素 mRNA 水平显著降低。在该结构中，与对照组相比，TGM'(rTon) 假手术和 TGM'(rTon) 心肌梗死组的 (P)RR mRNA 水平统计学上显著降低。然而，当我们比较 TGM'(rTon) MI 与 TGM'(rTon) 假手术组时，(P)RR mRNA 值显著增加。在 RV 中，TGM'(rTon) 假手术组的肾素 mRNA 水平与 C 假手术组相比显著降低。张力素过表达可能在心肌梗死期间调节 (P)RR mRNA 水平。
