大鼠淋巴因子控制未致敏淋巴细胞的迁移。

Rat lymphokines control the migration of nonsensitized lymphocytes.

作者信息

McFadden R G, Vickers K E

机构信息

Lawson Research Institute, St. Joseph's Health Centre, London, Ontario, Canada.

出版信息

Cell Immunol. 1989 Feb;118(2):345-57. doi: 10.1016/0008-8749(89)90383-3.

DOI:10.1016/0008-8749(89)90383-3

PMID:2910503

Abstract

We investigated whether mediators released from rat splenic mononuclear cells could control the in vitro migration of nonsensitized resting rat lymphocytes. Rat splenocytes stimulated with concanavalin A, other mitogens, or histamine release three lymphokines that alter rat lymphocyte migration. A positive chemokinetic factor, termed lymphocyte chemoattractant factor (LCF), has a molecular weight (MW) between 50 and 70 kDa. Two negative chemokinetic lymphokines can also be identified; lymphocyte migration inhibitory factor (LyMIF, MW 25-45 kDa) and a high MW inhibitor (HWMI, MW greater than 70 kDa). Lymphokines were destroyed by heat as well as by treatment with neuraminidase and trypsin. The action of LCF and LyMIF was prevented by phenylmethylsulfonylfluoride, a specific serine esterase inhibitor, and the action of LyMIF was also blocked by alpha-L-fucose. The discovery of these mediators provides the opportunity to study the importance of such chemokinetic lymphokines in animal models of disease.

摘要

我们研究了大鼠脾单核细胞释放的介质是否能够控制未致敏的静止大鼠淋巴细胞的体外迁移。用伴刀豆球蛋白A、其他促有丝分裂原或组胺刺激大鼠脾细胞会释放三种改变大鼠淋巴细胞迁移的淋巴因子。一种名为淋巴细胞趋化因子（LCF）的正向化学动力学因子，其分子量（MW）在50至70 kDa之间。还可鉴定出两种负向化学动力学淋巴因子；淋巴细胞迁移抑制因子（LyMIF，MW 25 - 45 kDa）和一种高分子量抑制剂（HWMI，MW大于70 kDa）。淋巴因子可被加热以及用神经氨酸酶和胰蛋白酶处理所破坏。苯甲基磺酰氟（一种特异性丝氨酸酯酶抑制剂）可阻止LCF和LyMIF的作用，α-L-岩藻糖也可阻断LyMIF的作用。这些介质的发现为研究此类化学动力学淋巴因子在疾病动物模型中的重要性提供了机会。