Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustr. 3, 14195 Berlin, Germany; Fritz Haber Institute of the Max Planck Society, Faradayweg 4-6, 14195 Berlin, Germany.
Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustr. 3, 14195 Berlin, Germany; Fritz Haber Institute of the Max Planck Society, Faradayweg 4-6, 14195 Berlin, Germany.
Curr Opin Chem Biol. 2018 Feb;42:16-24. doi: 10.1016/j.cbpa.2017.10.021. Epub 2017 Nov 5.
Due to the existence of numerous isomers, the in-depth analysis of glycans represents a major challenge. Currently, the majority of glycans are analysed using mass spectrometry (MS)-based techniques, which can provide information on regioisomers but usually fail to differentiate stereoisomers. A promising approach to overcome this limitation is to implement ion mobility spectrometry (IMS) as an additional gas-phase separation dimension. This review highlights recent developments in which IM-MS was used as a tool for comprehensive glycan analysis or as rapid screening method for glycan feature analysis. Furthermore, we summarize a series of very recent investigations in which gas-phase spectroscopy is applied to study glycans and discuss the potential of the hyphenation between IM-MS and infrared (IR) spectroscopy as a future tool for glycomics and glycoproteomics.
由于存在众多的异构体，深入分析聚糖是一个重大挑战。目前，大多数聚糖都是通过基于质谱（MS）的技术进行分析，这些技术可以提供关于区域异构体的信息，但通常无法区分立体异构体。一种有前途的方法是将离子淌度谱（IMS）作为额外的气相分离维度来实施。这篇综述强调了最近的发展，其中 IM-MS 被用作全面聚糖分析的工具，或作为聚糖特征分析的快速筛选方法。此外，我们总结了一系列最近的关于气相光谱用于研究聚糖的研究，并讨论了将 IM-MS 和红外（IR）光谱联用作为聚糖组学和糖蛋白质组学未来工具的潜力。
