Kato T
Department of Biochemistry, University of Tokyo, Faculty of Medicine, Japan.
J Neurochem. 1989 Feb;52(2):636-40. doi: 10.1111/j.1471-4159.1989.tb09167.x.
Activities of choline acetyltransferase (ChAT) were microassayed in individual cell bodies of motor neurons, isolated from freeze-dried sections after autopsy of lumbar spinal cords from four patients with sporadic amyotrophic lateral sclerosis (ALS) and four control patients with nonneurological diseases. Numerous large neurons were found in the anterior horn at the early degeneration stage of ALS, but the cell bodies atrophied and decreased in number at the late advanced stage. The small, atrophied neurons were very fragile and were easily destroyed during the isolation procedure with a microknife. The average activity, expressed on a dry weight basis, of 58 ALS neurons was lower than that of 67 control neurons. The large, well-preserved neurons at the early nonadvanced stage had markedly lower ChAT activities than control neurons. The specific activity gradually increased with the progress of atrophy but did not return to the control level.
对4例散发性肌萎缩侧索硬化症（ALS）患者和4例非神经疾病对照患者腰椎脊髓进行尸检后，从冻干切片中分离出运动神经元的单个细胞体，对胆碱乙酰转移酶（ChAT）的活性进行了微量测定。在ALS早期退变阶段的前角发现了许多大神经元，但在晚期进展阶段，细胞体萎缩且数量减少。小的萎缩神经元非常脆弱，在使用微型刀进行分离过程中很容易被破坏。58个ALS神经元以干重为基础表示的平均活性低于67个对照神经元。早期未进展阶段保存完好的大神经元的ChAT活性明显低于对照神经元。比活性随着萎缩的进展逐渐增加，但未恢复到对照水平。
