Clinical Pharmacy Center, Department of Pharmacy, Chinese PLA General Hospital, Beijing 100853, China.
Division of Infectious Disease, Rhode Island Hospital, Alpert Medical School & Brown University, RI 02903, USA.
Future Microbiol. 2017 Dec;12:1497-1510. doi: 10.2217/fmb-2017-0084. Epub 2017 Nov 7.
To assess the function of Ngg1 in Candida albicans and reveal the role of NGG1 in the morphological conversion and virulence of C. albicans.
MATERIALS & METHODS: C. albicans NGG1 gene was deleted in the wild-type strain SC5314 and the function of Ngg1 was assessed by western blot analysis. The phenotypes and the virulence of the ngg1 mutants were examined. Microarray analysis was performed to explore the mechanism.
The ngg1 mutants attenuated acetylated histone H3, obviously reduced filamentous growth and showed significantly diminished pathogenicity in all the infection models.
This study suggested the histone acetyltransferase activity of C. albicans Ngg1 and revealed the important role of NGG1 in morphological conversion and virulence of C. albicans. [Formula: see text].
评估 Ngg1 在白色念珠菌中的功能，并揭示 NGG1 在白色念珠菌形态转换和毒力中的作用。
在野生型菌株 SC5314 中敲除白色念珠菌 NGG1 基因，通过 Western blot 分析评估 Ngg1 的功能。检测 ngg1 突变体的表型和毒力。进行微阵列分析以探讨其机制。
ngg1 突变体减弱了乙酰化组蛋白 H3，明显减少了丝状生长，并且在所有感染模型中致病性显著降低。
本研究表明白色念珠菌 Ngg1 具有组蛋白乙酰转移酶活性，并揭示了 NGG1 在白色念珠菌形态转换和毒力中的重要作用。[公式：见正文]。
