Tasnim Tasnova, Al-Mamun Mir Md Abdullah, Nahid Noor Ahmed, Islam Md Reazul, Apu Mohd Nazmul Hasan, Bushra Most Umme, Rabbi Sikder Nahidul Islam, Nahar Zabun, Chowdhury Jakir Ahmed, Ahmed Maizbha Uddin, Islam Mohammad Safiqul, Hasnat Abul
1 Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka, Bangladesh.
3 Department of Pharmacy, University of Asia Pacific, Dhaka, Bangladesh.
Tumour Biol. 2017 Nov;39(11):1010428317729270. doi: 10.1177/1010428317729270.
Lung cancer is one of the most frequently occurring cancers throughout the world as well as in Bangladesh. This study aimed to correlate the prognostic and/or predictive value of functional polymorphisms in SULT1A1 (rs9282861) and XRCC1 (rs25487) genes and lung cancer risk in Bangladeshi population. A case-control study was conducted which comprises 202 lung cancer patients and 242 healthy volunteers taking into account the age, sex, and smoking status. After isolation of genomic DNA, genotyping was done by polymerase chain reaction-restriction fragment length polymorphism method and the lung cancer risk was evaluated as odds ratio that was adjusted for age, sex, and smoking status. A significant association was found between SULT1A1 rs9282861 and XRCC1 rs25487 polymorphisms and lung cancer risk. In case of rs9282861 polymorphism, Arg/His (adjusted odds ratio = 5.06, 95% confidence interval = 3.05-8.41, p < 0.05) and His/His (adjusted odds ratio = 3.88, 95% confidence interval = 2.20-6.82, p < 0.05) genotypes were strongly associated with increased risk of lung cancer in comparison to the Arg/Arg genotype. In case of rs25487 polymorphism, Arg/Gln heterozygote (adjusted odds ratio = 4.57, 95% confidence interval = 2.79-7.46, p < 0.05) and Gln/Gln mutant homozygote (adjusted odds ratio = 4.99, 95% confidence interval = 2.66-9.36, p < 0.05) were also found to be significantly associated with increased risk of lung cancer. This study demonstrates that the presence of His allele and Gln allele in case of SULT1A1 rs9282861 and XRCC1 rs25487, respectively, involve in lung cancer prognosis in Bangladeshi population.
肺癌是全球以及孟加拉国最常见的癌症之一。本研究旨在探讨SULT1A1基因（rs9282861）和XRCC1基因（rs25487）功能多态性的预后和/或预测价值与孟加拉国人群肺癌风险之间的相关性。开展了一项病例对照研究，纳入202例肺癌患者和242名健康志愿者，并考虑了年龄、性别和吸烟状况。提取基因组DNA后，采用聚合酶链反应-限制性片段长度多态性方法进行基因分型，并将肺癌风险评估为经年龄、性别和吸烟状况调整后的比值比。研究发现SULT1A1 rs9282861和XRCC1 rs25487多态性与肺癌风险之间存在显著关联。就rs9282861多态性而言，与Arg/Arg基因型相比，Arg/His基因型（调整后的比值比=5.06，95%置信区间=3.05-8.41，p<0.05）和His/His基因型（调整后的比值比=3.88，95%置信区间=2.20-6.82，p<0.05）与肺癌风险增加密切相关。就rs25487多态性而言，Arg/Gln杂合子（调整后的比值比=4.57，95%置信区间=2.79-7.46，p<0.05）和Gln/Gln突变纯合子（调整后的比值比=4.99，95%置信区间=2.66-9.36，p<0.05）也与肺癌风险增加显著相关。本研究表明，SULT1A1 rs9282861和XRCC1 rs25487分别存在His等位基因和Gln等位基因与孟加拉国人群肺癌预后有关。
