Lee Yao, Kiupel Matti, Soboll Hussey Gisela
Department of Pathobiology & Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, 784 Wilson Rd, A13, East Lansing, MI, 48824, USA.
BMC Vet Res. 2017 Nov 6;13(1):313. doi: 10.1186/s12917-017-1240-z.
Dendritic cells (DCs) are professional antigen-presenting cells that have multiple subpopulations with different phenotypes and immune functions. Previous research demonstrated that DCs have strong potential for anti-viral defense in the host. However, viruses including alphaherpesvirinae have developed strategies to interfere with the function or maturation of DCs, causing immune dysfunction and avoidance of pathogen elimination. The goal of the present study was to isolate and characterize equine lung-derived DCs (L-DCs) for use in studies of respiratory viruses and compare their features with equine blood-derived DCs (B-DCs), which are currently used for these types of studies.
We found that L-DCs were morphologically similar to B-DCs. Overall, B-DCs demonstrated higher expression of CD86 and CD172α than L-DCs, but both cell types expressed high levels of MHC class II and CD44, as well as moderate amounts of CD163, CD204, and Bla36. In contrast, the endocytic activity of L-DCs was elevated compared to that of B-DCs. Finally, mononuclear cells isolated from lung (L-MCs), which are used as precursors for L-DCs, expressed more antigen-presenting cell-associated markers such as MHC class II and CD172α compared to their counterparts from blood.
Our results indicate that L-DCs may be in an earlier differentiation stage compared to B-DCs. Concurrent with this observation, L-MCs possessed significantly more antigen-uptake capacity compared to their counterparts from blood. It is likely that L-DCs play an important role in antigen uptake and processing of respiratory pathogens and are major contributors to respiratory tract immunity and may be ideal tools for future in vitro or ex vivo studies.
树突状细胞（DCs）是专业的抗原呈递细胞，具有多种具有不同表型和免疫功能的亚群。先前的研究表明，DCs在宿主抗病毒防御中具有强大潜力。然而，包括α疱疹病毒科在内的病毒已发展出干扰DCs功能或成熟的策略，导致免疫功能障碍并逃避病原体清除。本研究的目的是分离和鉴定马肺源性DCs（L-DCs），用于呼吸道病毒研究，并将其特征与目前用于此类研究的马血源性DCs（B-DCs）进行比较。
我们发现L-DCs在形态上与B-DCs相似。总体而言，B-DCs比L-DCs表现出更高的CD86和CD172α表达，但两种细胞类型均高表达MHC II类分子和CD44，以及中等量的CD163、CD204和Bla36。相比之下，L-DCs的内吞活性较B-DCs有所提高。最后，从肺中分离的单核细胞（L-MCs），用作L-DCs的前体，与血源性单核细胞相比，表达更多的抗原呈递细胞相关标志物，如MHC II类分子和CD172α。
我们的结果表明，与B-DCs相比，L-DCs可能处于更早的分化阶段。与此观察结果一致，L-MCs比血源性单核细胞具有明显更强的抗原摄取能力。L-DCs可能在呼吸道病原体的抗原摄取和加工中起重要作用，是呼吸道免疫的主要贡献者，可能是未来体外或离体研究的理想工具。
