Orio Lara P, Boschin Giovanna, Recca Teresa, Morelli Carlo F, Ragona Laura, Francescato Pierangelo, Arnoldi Anna, Speranza Giovanna
Department of Chemistry, University of Milan , via C. Golgi 19, I-20133 Milano, Italy.
Department of Pharmaceutical Sciences, University of Milan , via L. Mangiagalli 25, I-20133 Milan, Italy.
J Agric Food Chem. 2017 Dec 6;65(48):10482-10488. doi: 10.1021/acs.jafc.7b04522. Epub 2017 Nov 17.
A hemp seed protein isolate, prepared from defatted hemp seed meals by alkaline solubilization/acid precipitation, was subjected to extensive chemical hydrolysis under acid conditions (6 M HCl). The resulting hydrolysate was fractionated by semipreparative RP-HPLC, and the purified fractions were tested as inhibitors of angiotensin converting enzyme (ACE). Mono- and bidimensional NMR experiments and LC-MS analyses led to the identification of four potentially bioactive peptides, i.e. GVLY, IEE, LGV, and RVR. They were prepared by solid-phase synthesis, and tested for ACE-inhibitory activity. The IC values were GVLY 16 ± 1.5 μM, LGV 145 ± 13 μM, and RVR 526 ± 33 μM, confirming that hemp seed may be a valuable source of hypotensive peptides.
一种通过碱溶解/酸沉淀从脱脂大麻籽粕中制备的大麻籽分离蛋白，在酸性条件下（6 M盐酸）进行了广泛的化学水解。所得水解产物通过半制备反相高效液相色谱进行分离，纯化后的组分作为血管紧张素转换酶（ACE）抑制剂进行测试。一维和二维核磁共振实验以及液相色谱-质谱分析鉴定出四种潜在的生物活性肽，即GVLY、IEE、LGV和RVR。它们通过固相合成制备，并测试了ACE抑制活性。IC值分别为GVLY 16±1.5 μM、LGV 145±13 μM和RVR 526±33 μM，证实大麻籽可能是降压肽的宝贵来源。
