1 Department of Life Sciences, Institute for Multidisciplinary Research, University of Belgrade , Belgrade, Serbia .
2 Neurology Clinic, Clinical Center of Serbia, University of Belgrade , Belgrade, Serbia .
Antioxid Redox Signal. 2018 Jun 10;28(17):1570-1575. doi: 10.1089/ars.2017.7433. Epub 2017 Dec 12.
The monitoring of progression in amyotrophic lateral sclerosis (ALS) relies on clinical outcome measures that take months to interpret, such as revised ALS functional rating scale (ALSFRS-R) score, with no approved biomarkers. A number of clinical studies have documented the involvement of oxidative stress in ALS pathology. Pertinent to this, we propose to evaluate oxidation-reduction potential (ORP) of cerebrospinal fluid (CSF) as a potential indicator of ALS progression. The case-control study included 24 patients with neurological non-neurodegenerative disorders (controls) and 82 ALS patients with different degrees of disease (ALSFRS-R score: 21-47). ORP was significantly higher in ALS patients than controls. It was not dependent on age or gender. A strong negative correlation was found between ORP and ALSFRS-R score for all patients and patients with spinal onset. In other words, ORP increased with ALS progression. No correlation was found for the subset of patients with bulbar onset, most likely because of the physical distance between neurodegenerative loci and the site of CSF collection. These results lead to the hypothesis that ORP of CSF has a potential as monitoring biomarker in ALS, particularly in the cohort of patients with spinal onset. Antioxid. Redox Signal. 28, 1570-1575.
肌萎缩侧索硬化症（ALS）的进展监测依赖于需要数月时间才能解读的临床结果测量指标，例如修订后的肌萎缩侧索硬化功能评定量表（ALSFRS-R）评分，而没有经过批准的生物标志物。许多临床研究已经记录了氧化应激在 ALS 病理中的作用。与此相关，我们提出评估脑脊液（CSF）氧化还原电位（ORP）作为 ALS 进展的潜在指标。该病例对照研究纳入了 24 名患有神经非神经退行性疾病的患者（对照组）和 82 名具有不同疾病程度的 ALS 患者（ALSFRS-R 评分：21-47）。与对照组相比，ALS 患者的 ORP 明显更高。它与年龄或性别无关。对于所有患者和以脊髓起病的患者，都发现 ORP 与 ALSFRS-R 评分之间存在强烈的负相关。换句话说，ORP 随着 ALS 的进展而增加。对于以延髓起病的患者亚组，未发现相关性，这很可能是由于神经退行性病变部位与 CSF 采集部位之间的物理距离。这些结果导致了这样的假设，即 CSF 的 ORP 具有作为 ALS 监测生物标志物的潜力，特别是在以脊髓起病的患者队列中。抗氧化。氧化还原信号。28,1570-1575.
