Vivian L. Smith Department of Neurosurgery, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX, USA.
Department of Anesthesiology, Shengjing Hospital, China Medical University, Shenyang, China.
Neural Plast. 2017;2017:9182748. doi: 10.1155/2017/9182748. Epub 2017 Oct 8.
Propofol is an intravenous anesthetic that has been widely used in clinics. Besides its anesthetic effects, propofol has also been reported to influence the regulation of the autonomic system. Controversies exist with regard to whether propofol exposure is safe for pregnant women and young children. In this work, human-induced pluripotent stem cell- (hiPSC-) derived neural progenitor cells (NPCs) were treated with propofol at 20, 50, 100, or 300 M for 6 h or 24 h, and acute and subacute cell injury, cell proliferation, and apoptosis were evaluated. Comparison of genome-wide gene expression profiles was performed for treated and control iPSC-NPCs. Propofol treatment for 6 h at the clinically relevant concentration (20 or 50 M) did not affect cell viability, apoptosis, or proliferation, while propofol at higher concentration (100 or 300 M) decreased NPC viability and induced apoptosis. In addition, 20 M propofol treatment for 6 h did not alter global gene expression. In summary, propofol treatment at commonly practiced clinical doses for 6 h did not have adverse effects on hiPSC-derived NPCs. In contrast, longer exposure and/or higher concentration could decrease NPC viability and induce apoptosis.
异丙酚是一种临床上广泛应用的静脉麻醉剂。除了其麻醉作用外，异丙酚还被报道影响自主神经系统的调节。关于异丙酚暴露对孕妇和幼儿是否安全存在争议。在这项工作中，用人诱导多能干细胞（hiPSC）衍生的神经祖细胞（NPC）在 20、50、100 或 300μM 下处理 6 小时或 24 小时，评估急性和亚急性细胞损伤、细胞增殖和细胞凋亡。对处理和对照 iPSC-NPC 进行全基因组基因表达谱比较。在临床相关浓度（20 或 50μM）下处理 6 小时，异丙酚处理不会影响细胞活力、凋亡或增殖，而更高浓度（100 或 300μM）的异丙酚降低 NPC 活力并诱导凋亡。此外，20μM 异丙酚处理 6 小时不会改变全局基因表达。总之，6 小时内以临床常用剂量处理异丙酚对 hiPSC 衍生的 NPC 没有不良影响。相比之下，更长的暴露时间和/或更高的浓度可能会降低 NPC 的活力并诱导凋亡。
