Thornton S N, Parrott R F
A.F.R.C. Institute of Animal Physiology and Genetics Research, Cambridge Research Station, UK.
Acta Endocrinol (Copenh). 1989 Jan;120(1):50-4. doi: 10.1530/acta.0.1200050.
Ovariectomized ewes (N = 7) were dehydrated for 24 h and then given iv injections of saline vehicle or 8 or 64 mg naloxone hydrochloride in saline. Blood samples were taken by jugular venepuncture before and after dehydration and at intervals during the 90 min period directly following naloxone treatment. Plasma concentrations of AVP, OT and cortisol were measured by radioimmunoassay. Plasma AVP levels and osmolality increased with dehydration, OT concentrations showed no consistent change, and cortisol levels were unaffected. After administration of naloxone, AVP and OT concentrations did not alter. The sampling procedure increased plasma cortisol levels and the duration of this response was prolonged by the 64 mg dose of naloxone.
切除卵巢的母羊（N = 7）脱水24小时，然后静脉注射生理盐水或含8毫克或64毫克盐酸纳洛酮的生理盐水。在脱水前后以及纳洛酮治疗后的90分钟内每隔一段时间通过颈静脉穿刺采集血样。采用放射免疫分析法测定血浆中抗利尿激素（AVP）、催产素（OT）和皮质醇的浓度。脱水后血浆AVP水平和渗透压升高，OT浓度无一致变化，皮质醇水平未受影响。给予纳洛酮后，AVP和OT浓度未改变。采血过程增加了血浆皮质醇水平，64毫克剂量的纳洛酮延长了这种反应的持续时间。
