五聚体蛋白3基因变异与活动性肺结核风险

Pentraxin 3 Genetic Variants and The Risk of Active Pulmonary Tuberculosis.

作者信息

Sheneef Abeer, Hussein Mona T, Mohamed Tamer, Mahmoud Aida A, Yousef Laila M, Alkady Ola A

机构信息

Department of Medical Microbiology & Immunology, Faculty of Medicine, Sohag University, Sohag, Egypt.

Department of Chest Diseases & Tuberculosis, Faculty of Medicine, Sohag University, Sohag, Egypt.

出版信息

Egypt J Immunol. 2017 Jan;24(1):21-27.

PMID:29120574

Abstract

Tuberculosis is a major health problem worldwide. Genetic factors are considered important determinants of the host susceptibility to Mycobacterium tuberculosis. The aim of the current study was to evaluate the association between pentraxin 3 genetic variants and the susceptibility and severity of active pulmonary tuberculosis. The study included 100 patients with newly diagnosed pulmonary TB and 50 healthy controls. PTX3 plasma level was assayed using ELISA and PTX3 genotypes (rs2305619, rs3816527, rs1840680) were detected by real time PCR in all participants. PTX3 rs1840680 genotype (AA) and allele (A) were significantly higher in the study group while the genotype (GG) was higher in the control group. The plasma level of PTX3 was higher in the patients than controls (P < 0.0001). There is a strong association between PTX3 plasma level and the activity and severity of pulmonary TB. PTX3 rs1840680 genotype AA is associated with increased risk of active pulmonary TB.

摘要

结核病是全球主要的健康问题。遗传因素被认为是宿主对结核分枝杆菌易感性的重要决定因素。本研究的目的是评估五聚体3基因变异与活动性肺结核易感性及严重程度之间的关联。该研究纳入了100例新诊断的肺结核患者和50例健康对照。采用酶联免疫吸附测定法检测所有参与者的血浆PTX3水平，并通过实时聚合酶链反应检测PTX3基因分型（rs2305619、rs3816527、rs1840680）。研究组中PTX3 rs1840680基因型（AA）和等位基因（A）显著更高，而对照组中基因型（GG）更高。患者的血浆PTX3水平高于对照组（P < 0.0001）。PTX3血浆水平与肺结核的活动性及严重程度之间存在密切关联。PTX3 rs1840680基因型AA与活动性肺结核风险增加相关。