Daef Enas A, Makhlouf Nahed A, Ahmed Entsar H, Mohamed Ahmed I, Abd El Aziz Mohamed H, El-Mokhtar Mohamed A
Department of Microbiology & Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Department of Tropical Medicine & Gastroenterology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Egypt J Immunol. 2017 Jan;24(1):37-48.
Occult Hepatitis B infection (OBI), defined as the presence of serum HBV DNA without detectable HBsAg, can be classified into seropositive OBI [anti-HBc and/or anti-hepatitis B surface (anti- HBs) positive] and seronegative OBI (anti-HBc and anti- HBs negative). We examined the role of anti-HBc as a screening test for OBI in HCV patients with chronic liver diseases and evaluated the possible impact of OBI on liver disease progression. 90 patients with hepatitis C related chronic liver diseases (CLD) and negative for HBsAg were divided into three equal groups; chronic hepatitis, liver cirrhosis and hepatocellular carcinoma (HCC). Patients were tested for anti-HBc by ELISA and by PCR for S-gene. Total anti-HBc was found in 26 patients (28.9%). 8 patients (8.9%) had positive serum HBV DNA. Of these, 2 were positive for anti-HBc and 6 negative for anti-HBc. No correlation between OBI and severity of HCV related CLD was observed. In conclusion, as OBI was not associated with total anti-HBc, it is invaluable surrogate marker for OBI detection.
隐匿性乙型肝炎感染（OBI）定义为血清中存在乙肝病毒脱氧核糖核酸（HBV DNA）但检测不到乙肝表面抗原（HBsAg），可分为血清学阳性OBI（抗-HBc和/或抗乙肝表面抗体（抗-HBs）阳性）和血清学阴性OBI（抗-HBc和抗-HBs阴性）。我们研究了抗-HBc作为慢性肝病丙肝患者OBI筛查试验的作用，并评估了OBI对肝病进展的可能影响。90例丙型肝炎相关慢性肝病（CLD）且HBsAg阴性的患者被分为三组，每组人数相等：慢性肝炎、肝硬化和肝细胞癌（HCC）。通过酶联免疫吸附测定（ELISA）检测患者的抗-HBc，并通过S基因聚合酶链反应（PCR）检测。26例患者（28.9%）检测到总抗-HBc阳性。8例患者（8.9%）血清HBV DNA阳性。其中，2例抗-HBc阳性，6例抗-HBc阴性。未观察到OBI与丙肝相关CLD严重程度之间的相关性。总之，由于OBI与总抗-HBc无关，它不是用于检测OBI的有价值替代标志物。
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