白细胞介素-8基因多态性及表达与埃及肝硬化患者肝细胞癌的关联
Association of Interleukin-8 Gene Polymorphisms and Expression with Hepatocellular Carcinoma in Egyptian Patients with Liver Cirrhosis.
作者信息
Hassanin Omayma M, El-Masry Tarek M, El-Baz Ahmed, Adel-Abou-Zaghla Heba M
机构信息
Department of Molecular, Ain-Shams Research Institute, Ain Shams University, Cairo, Egypt.
Department of Clinical Pathology, Ain Shams Hospitals , Ain Shams University, Cairo, Egypt.
出版信息
Egypt J Immunol. 2017 Jan;24(1):143-152.
Hepatocellular Carcinoma?(HCC) is one of the most common cancer in Egypt. Interleukin-8 Single Nucleotide Polymorphisms (-251 and +781 SNPs) are proposed genes for cancer susceptibility. Our aim was to evaluate the association of these two IL-8 SNPs polymorphisms and IL-8 gene expression with HCC in Egyptian patients with liver cirrhosis. IL-8 -251 and + 781 SNPs were assayed by TaqMan technique and IL-8 gene Expression study was done by Syber green method in 50 patients with liver cirrhosis (without HCC n=25and with HCC n=25)and 50 healthy controls. The expression of IL8 was significantly high in all patients compared to controls (P = 0.001), and higher in cirrhosis patients with HCC than cirrhosis without HCC (P = 0.003). IL-8(-251) A/A genotype and A allele were significantly higher in HCC patients (P = 0.005 and 0.009 respectively), while IL-8(+781) homozygote T/T was significantly low in HCC patients (P= 0.04). Finally, haplotype A-251/C+781 were associated with HCC.
肝细胞癌（HCC）是埃及最常见的癌症之一。白细胞介素-8单核苷酸多态性（-251和+781单核苷酸多态性）被认为是癌症易感性的相关基因。我们的目的是评估这两种白细胞介素-8单核苷酸多态性以及白细胞介素-8基因表达与埃及肝硬化患者肝细胞癌的相关性。采用TaqMan技术检测白细胞介素-8 -251和+781单核苷酸多态性，并采用Syber green法对50例肝硬化患者（无肝细胞癌患者n = 25，有肝细胞癌患者n = 25）和50例健康对照者进行白细胞介素-8基因表达研究。与对照组相比，所有患者的白细胞介素-8表达均显著升高（P = 0.001），且有肝细胞癌的肝硬化患者的白细胞介素-8表达高于无肝细胞癌的肝硬化患者（P = 0.003）。肝细胞癌患者中白细胞介素-8（-251）A/A基因型和A等位基因显著更高（分别为P = 0.005和0.009），而肝细胞癌患者中白细胞介素-8（+781）纯合子T/T显著更低（P = 0.04）。最后，单倍型A-251/C+781与肝细胞癌相关。
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