PFI1785w：一种与妊娠相关疟疾相关的高度保守蛋白。
PFI1785w: A highly conserved protein associated with pregnancy associated malaria.
作者信息
Kamaliddin Claire, Salnot Virginie, Leduc Marjorie, Ezinmegnon Sem, Broussard Cédric, Fievet Nadine, Deloron Philippe, Guillonneau François, Bertin Gwladys I
机构信息
MERIT, IRD, Université Paris 5, Sorbonne Paris Cité, Paris, France; Laboratoire d'Excellence GR-Ex; DHU Risques et grossesse; Paris, France.
3P5 Proteomic facility, COMUE Sorbonne Paris Cité, Université Paris Descartes, Paris, France.
出版信息
PLoS One. 2017 Nov 9;12(11):e0187817. doi: 10.1371/journal.pone.0187817. eCollection 2017.
Pregnancy-associated malaria (PAM) is one of the severe forms of Plasmodium falciparum infection. The main antigen VAR2CSA is the target of vaccine development. However, the large size of VAR2CSA protein and its high degree of variability limit to the efficiency of the vaccination. Using quantitative mass spectrometry method, we detected and quantified proteotypic peptides from 5 predicted PAM associated proteins. Our results confirmed that PFI1785w is over-expressed in PAM samples. Then, we investigated PFI1785w variability among a set of parasite samples from various endemic areas. PFI1785w appear to be more conserved than VAR2CSA. PFB0115w, another PAM associated protein, seems also associated with the pathology. Further vaccination strategies could integrate other proteins in addition to the major VAR2CSA antigen to improve immune response to vaccination.
妊娠相关疟疾（PAM）是恶性疟原虫感染的严重形式之一。主要抗原VAR2CSA是疫苗开发的靶点。然而，VAR2CSA蛋白的大尺寸及其高度变异性限制了疫苗接种的效率。使用定量质谱法，我们检测并定量了来自5种预测的PAM相关蛋白的蛋白型肽段。我们的结果证实，PFI1785w在PAM样本中过度表达。然后，我们研究了来自不同流行地区的一组寄生虫样本中PFI1785w的变异性。PFI1785w似乎比VAR2CSA更保守。另一种PAM相关蛋白PFB0115w似乎也与病理学有关。进一步的疫苗接种策略除了主要的VAR2CSA抗原外，还可以整合其他蛋白，以改善对疫苗接种的免疫反应。
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