Watson P D, Wolf M B
Department of Physiology, University of South Carolina, School of Medicine, Columbia 29208.
Am J Physiol. 1989 Jan;256(1 Pt 2):H186-94. doi: 10.1152/ajpheart.1989.256.1.H186.
The maximum value of capillary filtration coefficient (CFC) in maximally vasodilated cat skeletal muscle is disputed. It was hypothesized that the wide range of reported values was caused by the inability of gravimetric and volumetric measurements of tissue volume to separate transcapillary filtration from vascular volume changes. Consequently, we developed a method of measuring filtration rates from changes in venous protein concentration using Evan's blue-labeled albumin in the isolated hindlimb (pentobarbital sodium anesthesia). The filtration coefficient (PFFC) calculated from these filtration rates after a step in venous pressure should not be influenced by vascular volume changes. When the perfusate flow rate through the hindlimb was greater than 15 ml.min-1.100 g muscle-1, PFFC was 0.0085 +/- 0.0015 (SD, n = 8) ml.min-1.mmHg-1.100 g muscle-1. PFFC was observed to be unvarying from 1 to 12 min after the venous pressure elevation, in contrast to CFC values, which fall during the same period. It is argued that the difference between CFC and PFFC values is caused by vascular volume changes.
最大血管舒张状态下猫骨骼肌的毛细血管滤过系数（CFC）的最大值存在争议。有人提出，所报道的数值范围较宽是由于组织体积的重量法和容量法测量无法将跨毛细血管滤过与血管容积变化区分开来。因此，我们开发了一种利用伊文思蓝标记白蛋白，通过测量离体后肢（戊巴比妥钠麻醉）静脉蛋白浓度变化来测定滤过率的方法。静脉压阶跃后根据这些滤过率计算出的滤过系数（PFFC）不应受血管容积变化的影响。当通过后肢的灌注液流速大于15 ml·min⁻¹·100 g肌肉⁻¹时，PFFC为0.0085±0.0015（标准差，n = 8）ml·min⁻¹·mmHg⁻¹·100 g肌肉⁻¹。与CFC值在静脉压升高后1至12分钟内下降不同，观察到PFFC在此期间保持不变。有人认为CFC和PFFC值之间的差异是由血管容积变化引起的。
