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酵母中麦芽糖代谢全局阻遏物 Tup1 的功能分析：功能域的不同作用。

Functional analysis of the global repressor Tup1 for maltose metabolism in Saccharomyces cerevisiae: different roles of the functional domains.

机构信息

Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China.

College of Food Science and Technology, Hainan University, Haikou, 570228, China.

出版信息

Microb Cell Fact. 2017 Nov 9;16(1):194. doi: 10.1186/s12934-017-0806-6.

DOI:10.1186/s12934-017-0806-6
PMID:29121937
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5679332/
Abstract

BACKGROUND

Tup1 is a general transcriptional repressor of diverse gene families coordinately controlled by glucose repression, mating type, and other mechanisms in Saccharomyces cerevisiae. Several functional domains of Tup1 have been identified, each of which has differing effects on transcriptional repression. In this study, we aim to investigate the role of Tup1 and its domains in maltose metabolism of industrial baker's yeast. To this end, a battery of in-frame truncations in the TUP1 gene coding region were performed in the industrial baker's yeasts with different genetic background, and the maltose metabolism, leavening ability, MAL gene expression levels, and growth characteristics were investigated.

RESULTS

The results suggest that the TUP1 gene is essential to maltose metabolism in industrial baker's yeast. Importantly, different domains of Tup1 play different roles in glucose repression and maltose metabolism of industrial baker's yeast cells. The Ssn6 interaction, N-terminal repression and C-terminal repression domains might play roles in the regulation of MAL transcription by Tup1 for maltose metabolism of baker's yeast. The WD region lacking the first repeat could influence the regulation of maltose metabolism directly, rather than indirectly through glucose repression.

CONCLUSIONS

These findings lay a foundation for the optimization of industrial baker's yeast strains for accelerated maltose metabolism and facilitate future research on glucose repression in other sugar metabolism.

摘要

背景

Tup1 是一种普遍的转录抑制因子，能够协调控制酿酒酵母中不同基因家族的转录，这些基因家族受葡萄糖抑制、交配型和其他机制控制。已经鉴定出 Tup1 的几个功能域，每个功能域对转录抑制都有不同的影响。在这项研究中，我们旨在研究 Tup1 及其结构域在工业面包酵母中麦芽糖代谢中的作用。为此，在不同遗传背景的工业面包酵母中对 TUP1 基因编码区进行了一系列框内缺失，研究了麦芽糖代谢、发酵能力、MAL 基因表达水平和生长特性。

结果

结果表明，TUP1 基因对工业面包酵母中麦芽糖代谢是必需的。重要的是，Tup1 的不同结构域在工业面包酵母细胞的葡萄糖抑制和麦芽糖代谢中发挥不同的作用。Ssn6 相互作用、N 端抑制和 C 端抑制结构域可能在 Tup1 对 MAL 转录的调节中起作用，从而促进酵母对麦芽糖的代谢。缺少第一个重复的 WD 区域可能直接影响麦芽糖代谢的调节，而不是通过葡萄糖抑制间接影响。

结论

这些发现为优化工业面包酵母菌株以加速麦芽糖代谢奠定了基础，并为未来研究其他糖代谢中的葡萄糖抑制提供了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d8/5679332/412ec85c7190/12934_2017_806_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d8/5679332/580eaf22e55e/12934_2017_806_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d8/5679332/10300ccf1cde/12934_2017_806_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d8/5679332/d2431cae328a/12934_2017_806_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d8/5679332/b7f276749dec/12934_2017_806_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d8/5679332/ae7d6850d260/12934_2017_806_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d8/5679332/412ec85c7190/12934_2017_806_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d8/5679332/580eaf22e55e/12934_2017_806_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d8/5679332/10300ccf1cde/12934_2017_806_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d8/5679332/d2431cae328a/12934_2017_806_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d8/5679332/b7f276749dec/12934_2017_806_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d8/5679332/ae7d6850d260/12934_2017_806_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d8/5679332/412ec85c7190/12934_2017_806_Fig6_HTML.jpg

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